Detection of Bombyx mori nucleopolyhedrovirus from Rearing Soil Using PCR

doi:10.11924/j.issn.1000-6850.casb15020009

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (20): 14-17.doi: 10.11924/j.issn.1000-6850.casb15020009

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Detection of Bombyx mori nucleopolyhedrovirus from Rearing Soil Using PCR

Tang Fenfen¹, Zhang Yonghong¹, Li Jun², Shao Yulan¹, Zhong Jian¹, Yang Daping¹, Bai Xingrong¹

(¹Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan 661100; ²Colleage of Life Science and Technology, Hong He University, Mengzi Yunnan 661100)

Received:2015-02-02 Revised:2015-04-27 Accepted:2015-05-14 Online:2015-07-28 Published:2015-07-28

Abstract

Abstract: Bombyx mori nucleopolyhedrovirus disease is the most serious disease among silkworm virus diseases. In order to prevent and control this disease, using soil representing as environment factor, feasibility of detecting pathogen BmNPV in the environment by PCR was discussed. High conserved gene of polyhedrin of BmNPV was used to design specific primers and BmNPV in soil could be detected successfully by PCR. No specific amplification was found from the other three lepidopteran-specific nucleopolyhedrovirus in soil by PCR, indicating that this method was highly specific. Sensitivity test showed that viral polyhedra number was 8.3×10⁵ in the soil detected by PCR, and was 100-fold of that detected by microscopy.

Tang Fenfen,Zhang Yonghong,Li Jun,Shao Yulan,Zhong Jian,Yang Daping and Bai Xingrong. Detection of Bombyx mori nucleopolyhedrovirus from Rearing Soil Using PCR[J]. Chinese Agricultural Science Bulletin, 2015, 31(20): 14-17.

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Detection of Bombyx mori nucleopolyhedrovirus from Rearing Soil Using PCR

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