Abstract: In order to investigate the effect of tobacco stalk ash in flue-cured tobacco planting, realize the waste utilization of tobacco stalk ash in flue-cured tobacco production, a field experiment was carried out by using tobacco stalk ash as raw material to form base fertilizer and topdressing in contrast with general base fertilizer and topdressing, the tobacco agronomic traits, tobacco quality and economic traits with different distributions and applications of tobacco stalk ash were evaluated. The results showed that: (1) using tobacco stalk ash could prolong flue-cured tobacco growing stage, and promote fully mature and dry matter accumulation of tobacco leaf; (2) using tobacco stalk ash could improve agronomic characters of flue-cured tobacco, the maximum leaf area increased by 11.3%-20.7% in dome duration compared with the control treatment; (3) using tobacco stalk ash could improve tobacco quality and yield, among all the treatments, T1 (base fertilizer: rape-seed cake 378 kg/hm², tobacco stalk ash 252 kg/hm², diammonium phosphate 212.55 kg/hm² and calcium nitrate 220.65 kg/hm²; topdressing: tobacco stalk ash 735 kg/hm² and urea 96.45 kg/hm²) had the best effect; compared with the control, the yield increased by 75.6 kg/hm², the pure income of tobacco grower increased by 5952.9 yuan /hm², 14.85% higher than that of the control. Accordingly, the treatment of using tobacco stalk ash as raw material to form base fertilizer and topdressing (T1) was the best way of tobacco stalk ash utilization in flue-cured tobacco production, suitable for application in Yongzhou tobacco area of Hunan.