Abstract: Soil carbon sink and soil physical and chemical properties are influenced by different crops and different rotation patterns. The author designed 6 rotation patterns over 5 years, in order to study the impact of rotation on soil physical and chemical properties, soil respiration and microbial biomass carbon. The results showed that after winter, due to the windiness, drought and less rainfall condition, the loss of soil moisture above 20 cm layer was serious because of the soil evaporation, and soil moisture increased with the increase of soil layer. Treatments S2 and S3 could significantly increase the capacity of retaining soil moisture after winter; after harvest, soil moisture of most treatments could reach 14% and maintain at 8%-10% after winter, while soil moisture of the other treatments dropped down to below 5%. At the same time, treatments S2 and S3 could keep soil moisture of different depths at a higher, uniform and stable level, the others lost moisture greatly. The soil surface temperature peak was in the early August. The surface temperatures of S3 and S7 (25℃ ) were significantly lower than that of the other treatments (30-35℃), but the temperature of S3 was higher than that of the others at 50 cm soil layer. The stability of the temperature of S3 was shown between the soil depths, as well as the whole growth period, with no variation with summer climate changing. The order of year soil respiration (soil microbial and plant root) was S9＜S2＜S3＜S4＜S8＜S10＜S1＜S5＜S6＜S7, crop rotation could reduce the loss of surface soil organic carbon mineralization significantly, and maintained soil carbon sequestration capacity. The study also showed that soil respiration was significantly correlated to soil surface temperature. In conclusion, oats-alfalfa intercropping 1 year, alfalfa 1 year, oats 1 year, potato 2 years and oats-alfalfa intercropping 1 year, alfalfa 1 year, potato 3 years could effectively increase the ability of soil carbon sequestration and improve soil properties.