Abstract: In order to select the artificial light which is fit to flue-cured tobacco space seedling, the light environment index of 4 different artificial lights were tested in black laboratory, the light intensity and uniformity were analyzed when the distance between light and test sensor was 20, 30 and 40 cm. The author set 4 flue-cured tobacco plant areas with different light intensities, the physiological and ecological indexes were analyzed. The results showed that: (1) the average light intensity order of 4 artificial lights was: red and blue LED ＞ full spectrum fluorescent lamp ＞ warm white LED ＞ three color fluorescent lamp; (2) when the distance between light and test sensor was 30 cm, the light uniformity was the best, followed by 20, 40 cm; (3) the red and blue LED lamp with the light intensity of 150 μmol/(m²·s) in plant area could obtain the best seedling quality. The results could offer theoretical proof for the artificial light scheme in flue-cured tobacco space seedling.