Research Progress of Laser Microdissection Technique for Plant

doi:10.11924/j.issn.1000-6850.casb15030215

Abstract

Abstract: Genesis, differentiation and physiological responses are the results of complex multi-factor regulation in plant tissue. To understand its mechanism at the molecular level, we need to analyze the metabolite and gene expression of related cells with a prerequisite of obtaining these cells precisely. Laser microdissection (LMD) ensures that pure targeted cell subgroups or even a single cell can be rapidly and accurately captured under a microscope, which solves the problem of cell heterogeneity in tissue successfully. This review summarized the general operation process of LMD technology, compared the merits and demerits of various working approaches. Furthermore, this article generalized the application effects of LMD in researches of plant molecular biology, proteomics, metabolomics, pointed out certain existing issues and the future application prospect.

Chen Binli,Wang Yingqi and Zheng Caixia. Research Progress of Laser Microdissection Technique for Plant[J]. Chinese Agricultural Science Bulletin, 2015, 31(18): 121-125.

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