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Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (16): 190-193.doi: 10.11924/j.issn.1000-6850.casb15030260

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Identification of Root Rot Pathogen Paeonia ostii T.Hong et J.X.Zhang in Bozhou

Cheng Lei1,2, Fang Chengwu1,2, Chen Na1,2, Wang Jing1, Ye Shengming1, Wu Jihua3   

  1. (1School of Pharmacy, Bozhou Vocational and Technical College, Bozhou Anhui 236800;2Bozhou Institute of Chinese Medicine, Anhui academy of Chinese Medicine, Bozhou Anhui 236800;3Anhui Branch of Beijing Tongrentang TCMC, Tongling Anhui 244000)
  • Received:2015-03-31 Revised:2015-04-16 Accepted:2015-04-21 Online:2015-07-27 Published:2015-07-27

Abstract: The aim was to identify the pathogen species of root rot on Paeonia ostii T.Hong et J.X.Zhang in Bozhou City, Anhui Province. The symptoms of root rot on Paeonia ostii T.Hong et J.X.Zhang were observed and the pathogen was isolated, purified and cultured. Identification of the pathogen species was done according to the morphological characteristics, pathogenicity and rDNA-ITS sequence. One kind of purified strain was obtained, which was similar to the reported Fusarium solani. The symptoms of the healthy Paeonia ostii T.Hong et J.X.Zhang root induced by inoculation with the pathogen isolate were like the symptoms in the field. Homology BLAST comparison was conducted in GenBank, the rDNA-ITS sequences were clustered into one group with four login Fusarium solani and the login ID was HQ176440.1, EU327190.1, HQ839783.1, JX524023.1, respectively. The results demonstrated that root rot on Paeonia ostii T.Hong et J.X.Zhang was caused by Fusarium solani.