Abstract: In order to efficiently use okra germplasm resources and provide a theoretical basis for breeding new varieties, genetic diversity and correlation of 31 botanical and biological characteristics were investigated and evaluated with 60 accessions of okra germplasm resources. The results were as follows: (1) some of botanical characteristics, including stem color, stem epidermis, leaf color and fruit color, exhibited significant correlation with each other, the seed shapes of different taxa were distinguishable dependent on the phenotypic traits, the seeds collected from plants with erect, lobed or parted leaves and smaller corolla displayed round shape, whereas the seeds of plants with opposite phenotypic traits showed predominantly oblate shape; (2) the biological characteristics were highly diverse among okra germplasm resources, the variation coefficients from high to low were seed yield (72.51%), fruit number (43.84%), petiole length (40.84%), fruit length (36.95%), days to blossom (28.22%), leaf length (22.69%), leaf width (21.28%), branch number (21.05%), plant height (20.99%), flowering period (18.14%), germination days (16.96%), life cycle (8.97%) and petal number (2.57%); (3) there was a significant correlation of biological characteristics in okra germplasm resources, the seed yield showed extremely and significantly positive correlation with petiole length, fruit number, leaf length, leaf width, and flowering period, and their correlation coefficients were 0.53, 0.49, 0.46, 0.39 and 0.37, respectively. Study on genetic diversity of okra germplasm resources is of great significance in identification, evaluation and utilization of okra germplasm resources.