An Examination of the Bioactivities of Seven Wood-rotting Fungi

doi:10.11924/j.issn.1000-6850.casb15040084

Abstract

Abstract: To investigate and utilize the resource of wood associated fungi and its bioactive compounds, the biological activities of seven wood-rotting fungi were screened. These wood-rotting fungi were grown on the rice solid medium. The ethyl acetate extracts were prepared and thin layer chromatography (TLC)-bioautography-MTT assay, serial dilution method in 96 well plate and TLC-DPPH assay were adopted to evaluate the antibacterial activities and antioxidant activities, respectively. Results showed that, the ethyl acetate extracts of Pleurotus ostreatus specifically inhibited the growth of the Gram-positive bacteria Bacillus subtilis with minimum inhibitory concentration (MIC) value of 4.1 mg/mL, R_fvalue of 0.85. Meanwhile, the growth of the Gram-negative bacteria Escherichia coli was inhibited only by the ethyl acetate extracts of Fomitopsis pinicola with MIC value of 1.03 mg/mL, R_f value of 0.65. Besides the antibacterial properties, three components of the extracts of F. pinicola also displayed strong DPPH radical scavenging activity. Weaker antioxidant potentials were observed in the single component of the extracts of Piptoporus betulinus, Fomes fomentarius, Trametes suaveolens. Other fungi had negative responses to the DPPH assay. The results will facilitate the utilization and development of the wood-rotting fungi resource.

CLC Number:

Q939.95

Han Xiao,Sun Xumei,Wang Luwen,Jiang Jurui and Meng Wei. An Examination of the Bioactivities of Seven Wood-rotting Fungi[J]. Chinese Agricultural Science Bulletin, 2015, 31(28): 141-145.

References

[1] Xu X F, Yan H D, Chen J, et al. Bioactive proteins from mushrooms[J]. Biotechnology Advances,2011(29):667-674.
[2] 李峻志,吴小杰,党永,等.大型真菌抗氧化活性物质的研究进展[J].食品与生物技术学报,2014,33(5):455-465.
[3] 池玉杰.东北林区64种木材腐朽菌木材分解能力的研究[J].林业科学,2001,37(5):107-112.
[4] Wolff E R S, Wisbeck E, Silveira M L L, et al. Antimicrobial and antineoplasic activity of Pleurotus ostreatus[J].Applied Biochemistry and Biotechnology,2008(151):402-412.
[5] 储平,戴清源,李婉珍,等.糙皮侧耳(Pleurotus ostreatus)抗菌蛋白Pl-09的分离纯化[J].南京大学学报:自然科学,2010,46(5):596-601.
[6] 汪建中,李艳如,龚华锐.平菇菌粗多糖的抗氧化活性研究[J].生物学杂志,2014,31(1):15-19.
[7] Lemieszek M K, Langner E, Kaczor J, et al. Anticancer effect of fraction isolated from medicinal birch polypore mushroom, Piptoporus betulinus (Bull.: Fr.) P. Karst. (Aphyllophoromycetideae): in vitro studies[J]. International Journal of Medicinal Mushrooms,2009,11(4):351-364.
[8] 何晓义,沈先荣,刘琼,等.木蹄复方体外抗肿瘤作用的实验研究[J].中国实验方剂学杂志,2013,19(3):188-191.
[9] 李旭阳,包海鹰.木蹄层孔菌子实体提取物对H₂₂荷瘤小鼠体内抗肿瘤活性的影响[J].菌物研究,2013,11(3):202-206.
[10] 刘量,郑维发,周守标.木蹄层孔菌乙醇提取物体内抗肿瘤活性及其对荷瘤鼠免疫功能的影响[J].徐州医学院学报,2007,27(8):497-499.
[11] 姜丽,包海鹰.木蹄层孔菌子实体提取物体外抑菌活性研究[J].中国食用菌,2011,30(2):39-43.
[12] 吴凌,张喜光,石陶圣,等.白囊耙齿菌多糖提取条件优化及其生物活性[J].时珍国医国药,2010,21(12):3094-3096.
[13] Szeto Y T, Lau P C, Kalle W, et al. Direct human DNA protection by Coriolus versicolor (Yunzhi) extract[J]. Pharmaceutical Biology,2013,51(7):851-855.
[14] Wu Z X, Pang S F, Liu J J, et al. Coriolus versicolor polysaccharides enhance the immune response of crucian carp (Corassius auratus gibelio) and protect against Aeromonas hydrophila[J]. Journal of Applied Ichthyology,2013(29):562-568.
[15] Scarpari M, Parroni A, Zaccaria M, et al. Trametes versicolor bioactive compounds stimulate Aspergillus flavus antioxidant system and inhibit aflatoxin synthesis[J].Plant Biosystems,2015, DOI: 10.1080/11263504.2014.981235.
[16] 圣志存,郝利民,陶如玉,等.红缘拟层孔菌发酵物体外抗氧化活性的研究[J].食品工业科技,2014,35(13):129-133.
[17] 赵兴华,包海鹰,崔宝凯.红缘拟层孔菌化学成分与药理活性的研究概述[J].菌物研究,2010,8(2):119-124.
[18] 陈先晖,闫浩,戈群妹,等.红缘拟层孔菌醇提物弱极性成分分析及抗肿瘤、抗氧化活性[J].江苏农业科学,2011(1):356-358.
[19] 袁小红,张春杰,刘霞,等.6种高等真菌醇提取物对植物病原菌的抗菌活性研究[J].安徽农业科学,2013,41(12):5289-5290,5294.
[20] 王少娟,王雅琪,宝丽,等.黄白侧耳大米固体发酵次级代谢产物分析[J].菌物学报,2013,32(6):1028-1033.
[21] 赵江林,徐利剑,黄永富,等.TLC-生物自显影-MTT法检测滇重楼内生真菌中抗菌活性成分[J].天然产物研究与开发,2008(20):28-32,51.
[22] Cuendet M, Hostettmann K, Potterat O, et al. Iridoid glucosides with free radical scavenging properties from Fagraea blumei[J]. Helvetica Chimica Acta,1997,80(4):1144-1152.
[23] Hearst R, Nelson D, McCollum G, et al. An examination of antibacterial and antifungal properties of constituents of Shiitake (Lentinula edodes) and Oyster (Pleurotus ostreatus) mushrooms[J]. Complementary Therapies in Clinical Practice,2009,15(1):5-7.
[24] Keller A C, Maillard M P, Hostettmann K. Antimicrobial steroids from the fungus Fomitopsis pinicola[J]. Phytochemistry,1996,41(4):1041-1046.
[25] Mau J, Lin H, Chen C. Antioxidant properties of several medicinal mushrooms[J]. Journal of Agricultural and Food Chemistry,2002,50(21):6072-6077.