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Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (24): 173-178.doi: 10.11924/j.issn.1000-6850.casb15040139

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Induced Mutation Breeding to Improve Nisin Production Ability by Lactococcus lactis

Ge Jingping1, Sun Yanyang1, Li Xinglin1, Gao Dongni1, Cheng Dandan1, Song Gang1, Ling Hongzhi1, Ping Wenxiang1.2   

  1. (1Key Laboratory of Microbiology, Life Science Department, Heilongjiang University, Harbin 150080;2Engineering Research Center of Agricultural Microbiology Technology, Harbin 150050)
  • Received:2015-04-17 Revised:2015-05-13 Accepted:2015-05-18 Online:2015-08-26 Published:2015-08-26

Abstract: In this research, the highly Nisin-producing mutant strain DU101 was obtained from HDBR-06 by series of mutagenetic treatment, UV mutagenesis, nitrosoguanidine mutation, diethyl sulfate mutagenesis and ultraviolet (UV) and diethyl sulfate composite mutagenesis. The activity of Nisin of DU101 was enhanced from 442 IU/mL to 1389IU/mL, which was 3.14 times as much as that of the initial strain HDBR-06. The subculture test proved that the hereditary character of strain DU101 was stable.