Genetic Transformation of ProDH Antisense Gene Expression Vector

doi:10.11924/j.issn.1000-6850.casb15050016

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (26): 78-83.doi: 10.11924/j.issn.1000-6850.casb15050016

Special Issue: 生物技术

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Genetic Transformation of ProDH Antisense Gene Expression Vector

Zhao Jingyi, Luo Dan, Zhang Xichun

(College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206)

Received:2015-05-05 Revised:2015-06-16 Accepted:2015-06-25 Online:2015-09-23 Published:2015-09-23

Abstract

Abstract: Proline dehydrogenase (ProDH) antisense gene expression vectors were imported to ‘Naiyun 2000’ and tobacco by agrobacterium mediated leaf disc method. The tomato leaf regeneration system was established and optimized. Tobacco genetic transformation system was established at the same time which had simple operation, short regeneration cycle and high conversion efficiency. The experiment got 49 tobacco resistant buds and 177 tomato resistant buds. Specific primer PCR amplification was done in the validation, and 9 PCR positive resistant buds were obtained, including 7 tobacco resistant buds and 2 tomato resistant buds. It proved that the exogenous gene was successfully integrated into the genome of tomato. The experiment could lay the foundation for further tomato transgenic engineer.

Zhao Jingyi,Luo Dan and Zhang Xichun. Genetic Transformation of ProDH Antisense Gene Expression Vector[J]. Chinese Agricultural Science Bulletin, 2015, 31(26): 78-83.

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Genetic Transformation of ProDH Antisense Gene Expression Vector

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