Abstract: This article studied the treatment effect of distilled water, GA₃ and PEG on the germination of Stipa glareosa seeds. The results showed that the absorption rate of the germination rate of S. glareosa seeds displayed a rising trend with the rising of water temperature under the treatments of different water temperatures, and the water absorption of the seeds soaked with the water of different temperatures was significantly different. GA₃ treatment could promote the seeds germination. The germination rate and germination potential under the treatment of 80 mg/L GA₃ solution were the highest, which were 54% and 48%, respectively. The germination reached the peak on the 7th day, and the germination period was effectively shortened. With the addition of PEG solution, the seed germination was induced at various degrees. The lower the PEG solution concentration was, the more significant the inducing effect was. When the PEG solution concentration was 5%, seed germination rate and germination potential were comparatively high, indicating that drought stress could induce the seed germination. In conclusion, S. glareosa seeds can adapt to the dry soil habitat at a certain degree.