Abstract: Seed germination characteristics at different temperatures (10, 15, 20, 25℃) and water conditions (10%, 15%, 20%, 25%) were studied to explore the suitable temperature and moisture condition, in order to provide basic information and improve seed germination rate of Glycyrrhiza uralensis Fisch. The germination delay, germination potential, germination rate and the moldy rate were tested as germination indictors. The results showed that the germination delay in treatments of (15℃, 20%), (20℃, 15%/25%) and (25℃, 20%/25%) were 3 d which was shorter than that in other treatments. The germination potentials in treatments of (25℃, 15%/20%/25%) were significantly higher than that in other treatments. It showed that Glycyrrhiza uralensis Fisch seeds geminated quickly at high temperature and high moisture condition. Seed germination rate reached the highest level which was 92.00% at (25℃, 20%). The moldy rate was lower in treatments of (25℃, 10%/20%) than that in others. The above indicators suggested that suitable temperature and moisture condition for seed germination of Glycyrrhiza uralensis Fisch were 25℃ and 20%.