Abstract: Three experiments were carried out to investigate the effects of water exchange frequency and artificial substrates on hatchery production of pearl oyster Pinctada maxima from April 10 to June 27 in 2013. Experiment I: Five groups, named as A₁, A₂, A₃, A₄ and A₀, were established to evaluate the effects of water exchange frequency on growth and survival of Pinctada maxima larvae. The water exchange in the A₁, A₂, A₃ and A₄ were conducted every 3, 6, 9 and 12 days. The A₀ was control group with water change daily. Experiment II: Four groups, named as B₁, B₂, B₃ and B₄ were established to evaluate the effects of artificial substrates types on the settlement of pediveliger larvae. The artificial substrates of B₁, B₂, B₃ and B₄ were nylon net, rough plastic sheets, smooth plastic sheets and shade-cloth. Experiment III: Four groups, named as C₁, C₂, C₃ and C₄ were established to evaluate the effects of colors of artificial substrates on the settlement of pediveliger larvae. The result showed that the water exchange frequency, substrate type and artificial substrate color had significantly effects on hatchery production of Pinctada maxima. Growth and survival of larvae reared at the water change every 6–9 days were the highest in experiment I. The numbers of Pinctada maxima spat on rough collectors and deep color collectors were the highest in experiment II and in experiment III. Water change every 6-9 days and rough and deep color (red and black) plastic sheets should be used for hatchery production of Pinctada maxima.