Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (4): 160-164.doi: 10.11924/j.issn.1000-6850.casb15060076

Special Issue: 生物技术

Previous Articles     Next Articles

The DNA Content and Chlorophyll Fluorescence Characteristics of Diploid and Tetraploid of Primula forbesii

Sun Xiaoguang1, Zhang Xiaoman2, Wang Zhigang2, Cao Feng2, Wang Qian2   

  1. (1Highway Administrative Office of Transportation Bureau of Baoding, Baoding Hebei 071000;2Agricultural University of Hebei, Baoding Hebei 071000)
  • Received:2015-06-14 Revised:2015-10-21 Accepted:2015-10-23 Online:2016-02-01 Published:2016-02-01

Abstract: The materials were Primula forbesii and autotetraploid Primula forbesii doubled by colchicin. The DNA content of teraploid and diploid was tested by using the flow cytometer analytic method. The chlorophyll fluorescence characteristics in teraploid and diploid were compared and studied. The results showed that: (1) the tetraploid DNA relative content was 2 times that of the diploid; (2) the tetraploid chlorophyll a and b, chlorophyll content and total carotenoids content was higher than that of the diploid; (3) the variation tendency of Fv/Fm, Fv/Fo and PI value were similar, Fm, Fv, Fv/Fm, Fv/Fo and PI of tetraploid were higher than that of diploid, but tetraploid Fo was less than that of the diploid. Therefore, tetraploid has better photosynthetic performance compared with diploid.