Study on Introduction of TaNHX2 Gene to Enhance Salt Tolerance of Populus sp.

doi:10.11924/j.issn.1000-6850.casb15060142

Abstract

Abstract: Zhonglinmeihe is an elite variety of Populus sp. which is fast-growing, and has high yield and high quality of timber. It is important for afforestation programs in the development of China’s western regions. To enhance salt tolerance and widen adaptability of Populus sp. in northwest China, TaNHX2 gene was introduced into the variety through Agrobacterium-mediated transformation with explants of leaf and petiole. Based on PCR and Southern blotting analyses, most transgenic plants showed one copy of insertion of the target gene. Under various concentrations of NaCl treatment, transgenic poplar plants exhibited obvious resistance to salt stress. All in all, transgenic poplar plants with salt tolerance could facilitate afforestation and achieve high yield in northwest China.

CLC Number:

S718.3

Wang Yixue,Du Jianzhong,Hao Yaoshan,Zhang Huanhuan,Qin Yongjun,Wu Jiahe and Sun Yi. Study on Introduction of TaNHX2 Gene to Enhance Salt Tolerance of Populus sp.[J]. Chinese Agricultural Science Bulletin, 2015, 31(34): 203-208.

References

[1] Apse M P, Aharon G S, Snedden W A, et al. Salt tolerance conferred by overexpression of a vacuolar Na⁺/H⁺ antiporter in Arabidopsis[J]. Science,1999,285:1256-1258.
[2] Gaxiola R A, Rao R, Sherman A, et al. The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast[J]. Proc Natl Acad Sci USA,1999,96:1480-1485.
[3] Rausch T, Kirsch M, Zhigang A. Salt stress responses of higher plants: the role of proton pumps and Na⁺/H⁺antiporters[J]. Journal of Plant Physiology,1996,148:425-433.
[4] Quintero F J, Blatt M R, Pardo J M. Functional conservation between yeast and plant endosomal Na⁺/H⁺ antiporters[J]. FEBS Lett,2000,471:224-228.
[5] Zhang H X, Blumwald E. Transgenic salt- tolerant tomato plants accumulate salt in foliage but not in fruit[J]. Nat Biotechnol,2001,19:765-768.
[6] Zhang H X, Hodson J N, Williams J P, et al. Engineering salt- tolerant Brassica plants: Characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation[J]. Proc Natl Acad Sci USA,2001,98:12832-12836.
[7] 王关林,方宏筠.植物基因工程原理与技术[M].北京:科学出版社,1998:514-515.
[8] 任小燕,杜建中,孙毅.转AhCMO基因玉米后代的获得及耐盐性鉴定[J].分子植物育种,2013,11(3):332-338.
[9] 张志良,瞿伟菁,李小方.植物生理学实验指导[M].高等教育出版社,2009:274-277.
[10] 高英,同延安,赵营,等.盐胁迫对玉米发芽和苗期生长的影响[J].中国土壤与肥料,2007(2):30-34．
[11] Munns R. Physiological processes limiting plant growth in saline soils: some dogmas and hypotheses[J]. Plant Cell Environment,1993,16(1):15-24.
[12] 康建宏,吴宏亮,黄灵丹.干旱预处理的玉米幼苗对逆境的交叉适应研究[J].干旱地区农业研究,2008,26(6):143-148.
[13] 陈述.转基因杨树耐盐性研究进展[J].安徽农学通报,2010,16(6):30-32.
[14] 陈彩霞,司瑞新,沈昕,等.转Na⁺/H⁺逆向转运蛋白(PeNhaD1)基因派间杂种110杨的获得[J].西北林学院学学报,2009,24(1):61-65.
[15] 孙伟博,邓大霞,杨立恒,等.南林895杨树GmNHX₁基因的转入及其耐盐性分析[J].福建农林大学学报,2014,43(1):34-38.
[16] 姜超强,郑青松,刘兆普,等.转AtNHX₁基因杨树Tr品系的耐盐性研究[J].植物生态学报,2010,34(5):563-570.
[17] 陈利红,张波,徐子勤.AtNHX₁基因对荞麦的遗传转化及抗盐再生植株的获得[J].生物工程学报,2007,23(1):51-60.
[18] 陈观平,王慧中,施农农,等.逆向转运蛋白与植物耐盐性关系研究进展[J].中国生物工程杂志,2006,26(5):101-106.
[19] 张丽君,程林梅,杜建中,等.导入TaNHX2基因提高了转基因普那菊苣的耐盐性[J].生态学报,2011,31(18):5264-5272.
[20] 王全伟,李新玲,张海玲,等.转Na⁺/H⁺逆向转运蛋白基因(nhaA)大豆植株的获得及耐盐性分析[J].分子植物育种,2010,8(4):701-707.
[21] 俞嘉宁,原江锋,杨建雄,等.TaNHX2基因植物表达载体的构建及在拟南芥中的功能分析[J].西北植物学报,2006,26(11):2250-2256.
[22] 王小丽,赵欣梅,孙毅,等.盐胁迫对转TaNHX2基因玉米幼苗生长的影响[J].华北农学报,2014,29(2):165-169.
[23] 王小丽,杜建中,孙毅,等.转BADH基因玉米植株的获得及其耐盐性分析[J].作物学报,2014,40(11):1973-1979.
[24] 安宝燕,罗琰,高新起.紫花苜蓿Na⁺/H⁺逆向转运蛋白基因在拟南芥中表达提高转基因植株的耐盐性[J].作物学报,2008,34(4):557-564.
[25] Lu S Y, Jin X Y, Li Y F. Antiporter gene from Hordum brevisubulatum (Trin.) link and its over expression in transgenic tobaccos[J]. Journal of Integrative Plant Biology,2005,47(3):343-349.