Cold and Drought Tolerance Identification of Hybrid Progenies of Dongxiang Wild Rice (Oryza rufipogon Griff.) in Early Growth Stage

doi:10.11924/j.issn.1000-6850.casb15080026

Abstract

Abstract: To obtain elite rice intermediate lines for further breeding of rice varieties with cold and drought tolerance, the cold and drought tolerance of the hybrid progenies derived from Dongxiang wild rice (Oryza rufipogon Griff.) and cultivated rice were studied. The germination rate, germination index and survival seedling rate were chosen as evaluation indexes and employed to identify cold tolerance under different temperatures. 8 morphological traits related to drought tolerance were analyzed to identify the drought tolerance at seedling stage. The results showed that strain M65 and M132 had obviously higher germination rate and germination index than other tested strains at low temperature 7-10℃, which indicated that strain M65 and M132 had comparatively stronger cold tolerance at germination period. Under low temperature treatment of 7℃ for 10 days (7℃/10d), strain M10 and M117 had relatively higher survival seedling rate of 72% and 81.25%, respectively. This indicated that strain M10 and M117 had stronger cold tolerance at the budburst period. Low temperature treatments of 7-10℃ and 7℃/10d were suggested for evaluation of cold tolerance at the germination and budburst period for Indica rice, respectively. The comprehensive drought tolerance D value of strain M61 and M117 were higher than that of others at seedling stage. It indicated that strain M61 and M117 had stronger drought tolerance at seedling stage. These tolerant strains identified could be used for further rice breeding for cold and drought tolerance and genetic studies on the tolerance mechanism of Dongxiang wild rice.

谢建坤, and 万勇. Cold and Drought Tolerance Identification of Hybrid Progenies of Dongxiang Wild Rice (Oryza rufipogon Griff.) in Early Growth Stage[J]. Chinese Agricultural Science Bulletin, 2016, 32(3): 8-15.

References

[1]周勇, 朱孝波, 袁华, 等. 水稻单片段代换系芽期和苗期耐冷性分析及耐冷性QTL鉴定[J]. 中国水稻科学, 2013, 27(4):381-388
[2]Koseki Maiko, Kitazawa Noriyuki, Yonebayashi Shoji, et al. Identi?cation and ?ne mapping of a major quantitative trait locus originating from wild rice, controlling cold tolerance at the seedling stage [J]. Mol Genet Genomics, 2010, 284:45–54
[3]韩龙植, 张三元. 水稻耐冷性鉴定评价方法[J]. 植物遗传资源学报, 2004, 5(1): 75-80
[4]李霞, 戴传超, 程睿, 等. 不同生育期水稻耐冷性的鉴定及耐冷性差异的生理机制[J]. 作物学报, 2006, 32(1): 76-83
[5]韩龙植, 曹桂兰, 芮钟斗, 等. 水稻芽期耐冷性与其他耐冷性的相关关系[J]. 作物学报, 2004, 30(10): 990-995
[6]韩龙植, 高熙宗, 朴钟泽. 水稻耐冷性遗传及基因定位研究概况与展望[J]. 中国水稻科学, 2002, 16(2): 193-198
[7]金铭路, 杨春刚, 余腾琼, 郭桂珍, 等. 中国水稻微核心种质不同生育时期耐冷性鉴定及其相关分析[J]. 植物遗传资源学报, 2009, 10(4):540-546
[8] Farooq M, Wahid A, Lee D J, et al. Advances in drought resistance of rice [J]. Crit Rev Plant Sci. 2009, 28: 199-217
[9]胡标林, 杨平, 万勇, 等. 东乡野生稻BILs群体苗期抗旱性综合评价及其遗传分析[J]. 植物遗传资源学报, 2013, 14( 2): 249-256
[10]李艳, 马均, 王贺正, 等. 水稻品种苗期抗旱性鉴定指标筛选及其综合评价[J]. 西南农业学报, 2005, 18( 3): 250-255
[11]Xie J K, Kong X L, Chen J, et al. Mapping of quantitative trait loci for fiber and lignin contents from an interspecific cross Oryza sativa × Oryza rufipogon [J]. Zhejiang Univ. Sci. B (Biomed Biotechnol), 2011, 12(7): 518-526
[12]He G M, Luo X J, Tian F, et al. Haplotype variation in structure and expression of a gene cluster associated with a quantitative trait locus for improved yield in rice [J]. Genom Res., 2006, 16: 618-626
[13]余守武, 万勇, 胡标林, 等. 东乡野生稻细胞质雄性不育育性恢复的遗传研究[J]. 分子植物育种, 2005, 3(6): 761-767
[14]余丽琴, 徐巧玲, 邱兵余, 等. 原、异位保存东乡野生稻主要农艺性状的比较研究[J]. 植物遗传资源学报, 2007, 8(1): 99-101
[15]Luo X J, Wu S, Tian F, et al. Identification of heterotic loci associated with yield-related traits in Chinese common wild rice (Oryza rufipogon Griff.) [J]. Plant Sci., 2011, 181(1): 14-22
[16]陈大洲, 肖叶青, 赵社香,等. 东乡野生稻苗期和穗期的耐寒性研究[J].江西农业学报.1996, (8): 1-6
[17]简水溶, 万勇, 罗向东, 等. 东乡野生稻苗期耐冷性的遗传分析[J]. 植物学报, 2011, 46(1): 21-27
[18]胡标林, 余守武, 万勇, 等. 东乡野生稻全生育期抗旱性鉴定[J]. 作物学报, 2007, 33(3): 426-433
[19]谢建坤, 胡标林, 万勇, 等. 东乡野生稻与栽培稻苗期抗旱性的比较研究[J]. 生态学报, 2010, 30(6): 665-1674
[20]陈小荣, 陈明, 贺浩华, 等. 协青早B//协青早B/东乡野生稻BC1F9群体低磷耐性鉴定及其生理机制[J]. 应用生态学报, 2011, 22(5): 1169-1174
[21]王相琴, 吴应祥, 余金荣. 杂交水稻种子发芽试验[J]. 江西农业科技, 2003, (1): 11-12
[22]宋广树, 孙忠富, 孙蕾, 等. 东北中部地区水稻不同生育时期低温处理下生理变化及耐冷性比较[J]. 生态学报, 2011, 31(13): 3788-3795
[23]邓光辉, 张再兴, 曾令祥, 等. 贵州稻种资源芽期耐冷性研究[J]. 西南农业学报, 1989, 2(2): 84-85
[24]杨洛淼, 王敬国, 刘化龙, 等. 寒地粳稻发芽期和芽期的耐冷性QTL定位[J]. 作物杂志，2014,6:44-51
[25]蒋向辉, 余显权, 赵福胜. 贵州地方耐冷水稻品种芽期和苗期耐冷性的相关性研究[J]. 西南农业学报, 2004, 17(2): 177-180
[26]陈惠查, 张再兴, 阮仁超, 等. 贵州稻种禾类种质资源耐冷性和抗旱性鉴定与评价利用[J]. 贵州农业科学. 1999, 27( 6): 38-40