Abstract: The study aims to screen out sugarcane variety with good comprehensive agronomic traits for variety upgrading and improving the structure of sugarcane varieties grown in Guangxi Province. Analysis and evaluation were carried out on the cane yield, the stability and adaptability of sucrose content and agronomic traits of new sugarcane varieties depending on the investigation data of the regional trial of Guangxi sugarcane varieties in 2013-2014, in order to screen out the best strains for new variety certification. The two-year new planting and one-year ratooning cane test results indicated that, variety ‘GT06-244’, ‘GT06-1721’, ‘GT07-994’, ‘LC05-136’ and ‘GT06-3283’ had good comprehensive characters. Their average cane yield increased by 20.20%, 10.59%, 8.94%, 3.15% and 1.60%, respectively, compared with that of ‘ROC22’ (the control), the average sucrose content increased by 0.16, 0.17, 0.46, 0.93 and 0.36 percentage points, respectively. Among that, ‘GT06-244’ showed had high yield, middle sucrose content, good stability and being very easily to disleave, and ‘GT06-1721’ had high yield, middle sucrose content, high resistance to disease and lodging. ‘GT07-994’ and ‘LC05-136’ had early maturing, high yield and sucrose content with good ratooning ability traits. ‘GT06-3283’ had middle yield and high sucrose content. The performance of all these strains accorded with the new sugarcane approval standard of Guangxi, and could be adopted for demonstration planting and popularization and application in suitable planting area in the future.