Abstract: The paper aims to explore an effective way for agricultural resource utilization of polluted river dredged sludge. A field experiment was conducted taking the dredged sludge from polluted rivers as the research object, and two levels of nitrogen application (120 kg/hm2 and 240 kg/hm2) were arranged to study the effects of dredged sludge application on rice nitrogen (N) concentration, uptake, allocation and use efficiency at different growth stages. The results showed that: (1) dredged sludge increased N concentration and N accumulation in rice plant over the season significantly; (2) dredged sludge significantly improved nitrogen allocation proportions in stems, but obviously decreased that in leaves and spikes of rice after the heading stage; (3) dredged sludge significantly decreased nitrogen-use efficiency for biomass (NUEp) over the season and grain yield (NUEg) at grain maturity, but there had no significant effect on the nitrogen harvest index (NHI); (4) the effect of interactions between dredged sludge×N was not observed for N uptake and utilization at most growth stages of rice. Finally, dredged sludge increased N concentration and N accumulation, but decreased NUEp and NUEg at most growth stages of rice.