Diversity of Root Nodule Endophytes from Elaeagnus angustifolia L. and Arbuscular Mycorrhizal Fungi from Rhizosphere in Saline-alkali Soil

doi:10.11924/j.issn.1000-6850.casb15090039

Abstract

Abstract: This study was designed to evaluate the diversity of endophytes in Elaeagnus angustifolia L. root nodules and AM fungi (AMF) in the rhizosphere, located in saline-alkali soil of Heilongjiang Province. Nodule slicing and wet sieve methods combined with molecular biology technology were used to separate, identify and analyze the endophytes and AMF. In total, 27 endophytic strains were isolated and identified, including 4 actinomycetes, 14 bacteria and 9 fungi. The diversity index of endophytic bacteria was the highest while that of the endophytic actinomycetes related to root nodule formation was the lowest. The dominance index and evenness index of Frankia sp. (H01) were both higher than that of others. In addition, 8 strains AM fungi belonging to 5 different genus were obtained in the rhizosphere. Glomus ontained 3 strains, Funneliformis contained 2 strains, Septoglomus contained 1 strain, Rhizophagus contained 1 strain, and Acaulospora contained 1 strain, respectively. Rhizophagus intraradices was the dominant AMF spore with 100% isolation frequency in all soil samples and its spore density was 48.5/50 g. As a result, R. intraradices could be used as an important candidate strain in the process of mycorrhizal seedling and nodulating cultivation of Elaeagnus angustifolia L..

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