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Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (6): 102-105.doi: 10.11924/j.issn.1000-6850.casb15090066

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Analysis of Flax Stem Microflora Community Diversity by Denaturant Gradient Gel Electrophoresis

Zhou Xiaohang1,2, Ge Jingping2, Ping Wenxiang2   

  1. (1Laboratory of Pathogens and Immunity of College of Basic Medicine, Mudanjiang Medical University, Mudanjiang Heilongjiang 157011; 2Key Laboratory of Microbiology of Heilongjiang Province, College of Life Science, Heilongjiang University, Harbin 150080)
  • Received:2015-09-16 Revised:2016-01-25 Accepted:2015-11-24 Online:2016-03-07 Published:2016-03-07

Abstract: Flax is an important economic crop in China. In order to improve the efficiency of retting, the authors used PCR-DGGE technique to study the flax stem microflora diversity, and optimized the best condition of this method. The results showed that, when PCR-DGGE was carried out using the gel recycling samples, the electrophoresis time was 200-220 min, modified gel gradient was 25%-55%, fixed time was about 12-15 min, dyeing time was 20 min, developing time was 5-10 min, the electrophoresis and separation results were good. The study clearly obtained the bacteria diversity in flax retting by optimizing the electrophoresis condition of DGGE. It could set a base for the use of PCR-DGGE to study the microflora community diversity of flax stem.