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Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (5): 28-32.doi: 10.11924/j.issn.1000-6850.casb15090074

Special Issue: 园艺

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Influence of Radix paeoniae Rubra Decoction on Secretion of Virulence Factors and Proliferation of Staphylococcus aureus

张鹏鹏,李增顺,宋繁,孙英健 and 穆祥   

  • Received:2015-09-18 Revised:2016-01-12 Accepted:2015-11-25 Online:2016-02-19 Published:2016-02-19

Abstract: To study the influence of Radix paeoniae Rubra decoction on the secretion of virulence factors (α- Hemolysin, HLα; staphylococcal enterotoxins) and the growth of Staphylococcus aureus in vitro, Staphylococcus aureus was treated with Radix paeoniae Rubra decoction at different concentrations. Then the bacterial growth curve was recorded. Western-blot was performed to investigate the content of HLα, staphylococcal enterotoxins A (SEA) and B (SEB) in the supernatant of bacterial culture solution. The results showed that Radix paeoniae Rubra decoction could not inhibit the proliferation of Staphylococcus aureus (ATCC29213 and ATCC43300) obviously when the concentration ranged from 0 to 10 mg/mL. However, it could decrease the secretion of HLα, SEA and SEB in a dose-dependent manner. The results indicated that there was a potential anti-virulence ingredient in Radix paeoniae Rubra decoction.