Abstract: In order to screen out optimal varieties which adapt to different harvest time of flue-cured tobacco in tobacco-rice rotation region of Changsha, variety comparative experiments were conducted with 15 late rice varieties as materials under two simulated harvest time of flue-cured tobacco, normal harvest and delay harvest. The results showed that: (1) Looked from whole duration, all varieties adapted to be planted as late rice after tobacco under sown on 18th June, while only Xiangfengyou 103, Longxiangyou 130, Shenyou 9520, Xiangfengyou No.9, Jinyou 284, Fengyuanyou 299 and Fengyuanyou 272 could mature normally under sown on 28th June. (2) Looked from yield, under sown on 18th June, yield of Liangyou 527, Fengyuanyou 272 and Longxiangou 130 could reach to about 8000 kg/hm2, which were the first choice of late rice variety after tobacco for tobacco-rice rotation region under normal harvest of tobacco leaves. Under sown on 28th June, Fengyuanyou 272, Xiangfengyou 103, Shenyou 9520 and Xiangfengyou No.9 were ideal choices, whose yield was above 7600 kg/hm2. (3) Relational analysis showed that very significantly positive correlation existed between yield and grain number per panicle, and positive correlation existed between yield and harvest index. So choosing late rice varieties with bigger panicle type and higher harvest index was easy to gain high yield. The results provide the guidance for late rice variety screening in Changsha tobacco area.