Abstract: Agronomic trait mutant library was established by the method of heavy ion irradiation of ‘Wuyunjing7’, amylose and protein mutant materials were screened and the correlation between grain quality and agronomic traits was analyzed. The results showed that the mutation range of amylose content was 11.50%-21.70% of 202 ‘Wuyunjing7’ mutants, 23 belonged to high amylose content mutants and 29 belonged to low amylose content mutants; the mutation range of protein content was 7.91%-13.97%, 29 high protein content mutants and 24 low protein content mutants were obtained. The results of correlation analysis showed that the protein content had a significantly negative correlation with grain number of per spike, plant height, setting rate, thousand kernel weight and panicle length, the correlation coefficient was -0.540, -0.593, -0.387, -0.581 and -0.563, respectively. A significantly positive correlation was found between the amylose content and the panicle length, and the correlation coefficient was 0.294. The principal component analysis showed that the 6 agronomic traits were integrated into 3 principal components, and the accumulative contribution rate reached 89.118%. The establishment of amylose and protein mutant lines could provide materials for the study of synthesis pathway of quality characters and gene regulation and quality breeding.