Abstract: The expression vector of NtFT2 sense gene was constructed to study the regulation effect of NtFT2 gene on the bud dormancy; and transgenic procedure was established to provide a foundation for gene transformation in Narcissus tazetta var. Chinensis. NtFT2 gene was cloned from buds by PCR, NtFT2 sense gene was inserted into plant expression vector pCAMBIA1301 with double GUS by PCR, double digestion and connection method to obtain expression vector P1301-FT2 with NtFT2 sense gene. Then recombinant plasmid P1301-FT2 was transformed into Agrobacterium through freeze-thawing method. NtFT2 sense gene was transformed into callus by Agrobacterium-mediated transformation method, genetic transformation efficiency was detected by GUS histochemical staining method. Results showed that: expression vector with NtFT2 sense gene was obtained; the best transient expression of GUS reached 64.28% under the conditions as follows: callus which were pretreated with 0.5 mol/L mannitol for 6 h; and the Agrobacterium suspension was with OD600 value of 1.0, callus were infected for 20 min, and then they were transferred onto medium for co-cultivation for 6 days at 25℃ in the dark. Expression vector with NtFT2 gene was constructed, and a high-efficiency transgenic procedure mediated by Agrobacterium tumefaciens with NtFT2 sense gene was developed from callus in Narcissus tazetta var. Chinensis.