Abstract: The interspecific associations of 16 dominant species of wetland plant communities in Yangguan Nature Reserve of Dunhuang were analyzed by using the χ2-test, Pearson’s correlation coefficients, Spearman’s rank correlation coefficients and cluster analysis, so as to provide scientific basis for the protection, management and restoration of the wetland. The results of multispecies overall association showed that negative correlations existed among the 16 dominant species, which suggested that the community structure was unstable; the interspecific correlations showed that 53 species- pairs had positive associations, while 67 species-pairs had negative associations, and the ratio of positive to negative correlations were 0.79 based on χ2-test; the Spearman’s correlation coefficient test was more sensitive than χ2-test and Pearson’s correlation coefficient test, and Spearman’s correlation coefficient test reflected there were 52 pairs which had positive correlations, 68 pairs had negative correlations and the positive/negative correlation coefficient ratio was 0.76. The results of the 3 tests showed that most of interspecific associations were not significant, and the negative associations were more than the positive associations, the wetland plant communities in Yangguan Reserve of Dunhuang were relatively fragile, community structure was simple and unstable, and were still under dynamic succession. According to the results of Spearman’s rank correlation, the 16 dominant species were classified into 3 ecological groups. Interspecific association analysis plays an important role in a comprehensive understanding of the relationship among dominant species of wetland plants in nature reserve and provides a theoretical basis for the ecological restoration and plant diversity protection in Yangguan wetland.