Abstract: The paper aims to analyze the variation characteristics of runoff in Ebinur Lake Basin, the variation law of runoff in Ebnur Lake was studied based on the observed monthly runoff of three key hydrological control stations during 1964-2012, and by using indexes such as non-uniformity coefficient, concentration degree and variation amplitude. The results indicated that: (1) the changing trend of annual runoff in Ebnur Lake Basin was “decrease-increase-decrease”, as a whole, it decreased slowly during 1964-1976, increased during 1977-2002, decreased during 2003-2012; the runoff was mainly concentrated in summer while it was the least in spring; (2) as time went on, the non-uniformity and concentration degree of annual runoff distribution of Jinghe River increased and that of Bortala River decreased; (3) the absolutely variation amplitude of annual runoff distribution of Jinghe River and Bortala River decreased at first, then increased with the time while the relatively variation amplitude of annual runoff distribution had a continuous decreasing trend.