Abstract: In order to discuss the salt resistance of four hybrid combinations of Fraxinus seedlings, 1-year-old hybrid seedlings of Fraxinus were used to study the physiological response to different salt concentrations (0‰, 4‰, 8‰ and 12‰). The results showed that No.6 hybrid seedling presented the highest salt resistance in four combinations. The growth indexes of all hybrid seedlings decreased with the increase of salt concentrations, but only slight decrease was measured in No.6 hybrid seedling. The electric conductivity increasing rate of No.6 hybrid seedling was the lowest and MDA content was also the lowest under salt stress among all the combinations, the activities of SOD and POD in leaves of No.6 hybrid seedling were significantly higher than that of others. Meanwhile, the increasing rate of Pro, soluble sugar and chlorophyll of No.6 hybrid seedling was significantly higher than that of others. No.6 hybrid seedling showed significantly higher activities of antioxidant enzyme, which could lower the damage of mesophyll cell, keep better integrity of cell membrane, lower the level of membrane lipid peroxidation and the loss of chlorophyll, increase photosynthesis and accumulate more photosynthetic product, thus this hybrid seedling had relatively quick plant growth and high salt resistance. This study might lay a theoretical foundation of salt-tolerant breeding of Fraxinus.