Molecular Mechanism of Galium aparine Resistance to Tribenuron-Methyl

doi:10.11924/j.issn.1000-6850.casb16020069

Abstract

Abstract: In recent years, the tribenuron-methylin resistant Galium aparine was serious throughout Henan Province, and common dose of tribenuron-methyl cannot control Galium aparine any more. The objective of this study is to understand the molecular mechanism of the Galium aparine resistance to tribenuron-methyl. Through total DNA extraction, ALS clone and sequencing, the authors found the specific mutation sites in amino acid sequence of acetolactate synthase (ALS) in the resistant biotype of Galium aparine. Compared with the susceptible biotype, there were three types of amino acid substitutions in the resistant biotypes, of which, the amino acid substitution of Pro197 (CCC) to Thr (ACC) and Pro197 (CCC) to Leu (CTC) were located in the highly conserved region Domain A, Thr 457 (ACC) to Ser (TCC) in the non- conservative region. The substitution of Pro197 might be the reason for the resistance in the R-biotype of Galium aparine to tribenuronmethyl.

CLC Number:

S481.4

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