Abstract: In order to explore the optimal nitrogen fertilizer application level for achieving high yield cultivation of‘Nongxiangyou676’, we conducted a randomized block experiment with N: P2O5: K2O=1:0.5:0.8, basal fertilizer ratio of 60%, 100% and 40%, and three different pure nitrogen application levels of 120, 150 and 180 kg/hm2, respectively. The effects of different fertilization levels on main agronomic characters and yield of‘Nongxiangyou 676’were studied. The results showed that, under the application of 150 kg/hm2 pure nitrogen, both SPAD and leaf area of the upper three leaves were the largest at full tillering stage and milky stage, the total grain number per panicle and the seed setting rate increased significantly. Therefore, ‘Nongxiangyou676’achieved the highest yield. It was concluded that‘Nongxiangyou676’had the best comprehensive characters and the highest yield per unit area with the application of 150 kg/hm2 pure nitrogen. Thus, the most appropriate nitrogen fertilizer application level of‘ Nongxiangyou676’was 150 kg/hm2.