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Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (28): 183-188.doi: 10.11924/j.issn.1000-6850.casb16030042

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Response Surface Methodology Optimizing Extraction Process of Oil from Panicum miliaceum L.

Tian Xiang, Qiao Zhijun   

  1. (Key Laboratory of Crop Gene Resources & Germplasm Enhancement on Loess Plateau, Ministry of Agriculture/ Shanxi Key Laboratory of Genetic Resources and Genetic Improvement of Minor Crops, Institute of Crop Germplasm Resources
  • Received:2016-03-04 Revised:2016-09-21 Accepted:2016-06-24 Online:2016-10-11 Published:2016-10-11

Abstract: In order to determine the optimum extraction process of oil fromPanicum miliaceum L., and analyze the fatty acid composition, based on the single factor experiment, oil yield was taken as the response target, central composite design of response surface methodology was used to optimize the extraction conditions, Soxhlet extraction method was used to extract oil. Gas chromatograph-mass spectrometer (GC-MS) was used to detect fatty acid composition of oil fromPanicum miliaceum L.. The results showed that taking petroleum ether as extraction solvent and making liquid-to-solid ratio at 20:1, extracting oil at the temperature of 80℃ and leaching for 30 min could get the best yield, which was 4.51%. GC-MS analysis showed that the main fatty acids of oil fromPanicum miliaceum L. were oleic acid, linoleic acid, palmitic acid and linolenic acid, which were unsaturated and high-quality fatty acids.