Advances in Tissue Culture and Genetic Transformation of Pear

doi:10.11924/j.issn.1000-6850.casb16030218

Abstract

Abstract: In order to summarize the research progress of pear tissue culture and genetic transformation, and lay the foundation for genetic improvement of pear in the future, this paper mainly analyzed the influence of medium types, the type and concentration of plant growth regulators on the process of tissue culture, such as shoot tips culture and leaves culture; discussed the reason of explants browning and countermeasures; summarized the foreign imported genes in genetic transformation of pear currently; analyzed the influence of genotype, antibiotics, and agrobacterium on the efficiency of pear genetic transformation; and finally summarized the identification method of transgenic pear plants. On the basis of previous studies, the current research mainly exist the following problems: (1) Restricted by genotype, the scope of pear tissue culture and genetic transformation research is still narrow; (2) The transformation frequency is not stable using agrobacterium-mediated genetic transformation method; (3) Genetic transformation of functional genes associated with quality and resistance is not deep enough. Therefore, it need to establish the highly efficient and stable regeneration and transformation system, and develop the more effective genetic transformation method, and accelerate the research of functional genes, thus expedite the genetic improvement of pear varieties in the future.

CLC Number:

S661.2

References

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