Abstract: Apple valsa canker is one of the most serious pathogenic fungi in north China which often leads to widespread death among apple trees. To control the fungal disease with biocontrol methods, tissues of apple trees infected by valsa canker were collected from farms located in Yu county, Yangquan city, Shanxi Province. A pure cultural fungal strain numbered as SX1408 was obtained using the tissue isolation method. A partial ITS sequence (GenBank Accession No. KR007599) was amplified using fungal universal primers ITS1 and TIS4. After online Blast analysis and MEGA5.0 cluster analysis, the strain SX1408 was identified as Valsa mali Miyabe et Yamada. Antagonistic study toward the strain SX1408 was conducted using the plate confrontation method. Among the 19 tested Streptomyces species kept in our lab, four of them manifested antagonistic activity towards the disease. Strain BUAS13-03 showed the highest antifungal activity with a diameter of inhibition zone of 2.80 cm and an inhibition ratio of 78%. The study provides certain references for biological control of apple valsa canker disease.