Abstract: The aim of this study is to explore the effect of boron fertilizer application on rice in boron deficiency soil in south China. Using field plot test, effects of different boron fertilizer application methods and amounts on the growth period, tillering, chlorophyll content of flag leaf, biomass and yield of rice were analyzed. The results showed that the basal application of boron fertilizer and combination of basal application and foliar application of boron fertilizer could make rice growth period early, especially full heading stage which was 1-3 days earlier. And they could also accelerate tillering, increase chlorophyll content of flag leaf, rice biomass and yield. In conclusion, conventional fertilization basal application of 11.25 kg/hm2 boron fertilizer and conventional fertilization basal application of 7.5 kg/hm2 boron fertilizer foliar application of 0.45 kg/hm2 boron fertilizer had relative better effect, and are worthy of popularization and application in rice production in south China.