BmNPV Affecting the Activity and Gene Expression of Phenoloxidase in Bombyx mori

doi:10.11924/j.issn.1000-6850.casb16050089

Chinese Agricultural Science Bulletin ›› 2016, Vol. 32 ›› Issue (32): 25-28.doi: 10.11924/j.issn.1000-6850.casb16050089

Special Issue: 生物技术

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BmNPV Affecting the Activity and Gene Expression of Phenoloxidase in Bombyx mori

Tang Fenfen^1,2, Yang Weike¹, Zhu Feng¹, Shao Yulan¹, Zhang Yonghong¹, Bai Xingrong¹

(¹Institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan 661101;²College of Plant Protection, Yunnan Agricultural University, Kunming 650201)

Received:2016-05-16 Revised:2016-11-02 Accepted:2016-06-20 Online:2016-11-15 Published:2016-11-15

Abstract

Abstract: In order to clarify the effect of BmNPV infection on the activity and gene expression of phenoloxidase in hemolymph of Bombyx mori, the real-time fluorescent quantitative PCR was applied to analyze the expression of prophenoloxidase gene PPO1 and PPO2 in hemolymph of the 5^th instar larvae of silkworm, meanwhile the activity of phenoloxidase was determined after feeding Bombyx mori nucleopolyhedrovirus. The results revealed that the expression of PPO1 and PPO2 presented an increasing trend after oral infected by BmNPV after 6 h and 9 h, but the relative expression quantity of PPO1 had significant difference with that of PPO2 at 6 h and 9 h. In addition, the phenoloxidase activities of the treatment group were significantly higher than that of the control group at 6 h and 9 h, while phenoloxidase activity of the treatment group was significantly lower at 24 h. As a kind of humoral immunity factor, phenoloxidase may be involved in the immune defense response of silkworm by BmNPV infection.

CLC Number:

S884.5
Q789

Tang Fenfen,Yang Weike,Zhu Feng,Shao Yulan,Zhang Yonghong and Bai Xingrong. BmNPV Affecting the Activity and Gene Expression of Phenoloxidase in Bombyx mori[J]. Chinese Agricultural Science Bulletin, 2016, 32(32): 25-28.

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BmNPV Affecting the Activity and Gene Expression of Phenoloxidase in Bombyx mori

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