Abstract: Taking the plant list of Daiyun Mountain National Nature as a research object, the pollination type of families for seed plants were counted based on APWeb and literatures. The main pollination type and their ancestral state of seed plants in Daiyun Mountain Nature Reserve were studied. The results showed that: there were 144 families being zoophily-related, 87.27% of total families, entirely non-biological pollination mode accounted for 12.12% of all families. For the single pollination modes of seed plants, the families of zoophily-related mode (124 families) were the largest, following by anemophily mode (14 families) and hydrophily mode (4 families). As for the mixed pollination mode of seed plants, the families of zoophily and anemophily mixed mode were the largest. The results showed that the zoophily-related mode played an important role in the pollination of seed plants in Daiyun mountain nature reserve. Meanwhile, there were 9 primitive families in Daiyun mountain nature reserve, which zoophily-related mode with 8 families, and zoophily and anemophily mixed mode with 1 family, which indicated that zoophily-related mode occupied a main position in the evolution of primitive angiosperms, and might be ancestral state of pollination mode for extant angiosperms in Daiyun Mountain. The results could provide a theoretic basis for understanding the development and evolution of seed plants in Daiyun mountain nature reserve.