Effects of Artificial Aging on Seed Vigor and Ultraweak Luminescence of Wheat seeds

doi:10.11924/j.issn.1000-6850.casb16060106

Abstract

Abstract: In order to ascertain the effect of artificial aging on ultraweak luminescence and the correlation between UWL and seed vigor, and explore a new way of seed vigor non-destructive testing, seeds of wheat variety ‘Jimai 22’ were used to study the changes of UWL intensity and its relationship to seed vigor and ATP content during artificial aging. The results show that with the extension of artificial aging time, the germination percentage, germination potential, germination index, UWL intensity and ATP content decreased gradually; after artificial aging for 48h, they were reduced by 19.19%、34.34%、38.78%、27.77% and 13.84% respectively as compared with the control. Correlation analysis indicated that the UWL intensity is significantly and positively correlated with germination percentage, germination potential, germination index and ATP content. It was concluded that UWL as a detection method of fast, nondestructive and sensitive can be applied in the study of seed vigor non-destructive testing.

CLC Number:

S330.3

References

[1] Abeles FB. Plant chemiluminescence[J].Ann Rev Plant PhysiolPlant Mol boil,1986,37:49-72.
[2] 张新华,杨洪强.植物的超微弱发光[J].山东农业大学学报(自然科学版),2003,34(4): 605-608.
[3] POPP F A.Biophoton emmission[J].Experientia,1988,44(7):543- 544.
[4] Hideg E,Bjorn L O.Ultraweak light emission, free radicals, chilling and light sensitivity[J].Physiol Plant,1996,98:223-228.
[5] Slawlnski J. Luminescence research and its relation to ultraweak cell radiation[J].Experiential, 1988,33(7):559-571.
[6] Rattemeyer M R, Popp F A, Nagl W, et al. Evidence of photon emission from DNA in living cells [J].Naturwissenschaften,1981,68:572-573.
[7] 毛大璋,沈恂,张月敬,等.代谢抑制剂对萌发绿豆超弱发光的影响[J].生物物理学报,1988,4(2):116-120.
[8] 谭石慈,邢达,唐永红,等.植物叶片超微弱发光光谱研究[J].光子学报,2000,29(11): 961-965.
[9] 张新华,李富军,申琳,等.超弱发光技术在植物逆境生理研究中的应用[J].植物生理学通讯,2009,45(9):931-935.
[10] Yan Y, Popp FA, Rothe GM. Correlation between germination capacity and biophoton emission of barley seeds (Hordeum vulgare L.) [J].Seed Sci Technol,2003,31(2):249-258.
[11] Costanzo E, Gulino M, Lanzano L,et al.Single seed viability checked bydelayed luminescence [J] .Euro Biophys J, 2008, 37(2): 235-238.
[12] 张新华,杨洪强,李富军.水分胁迫下苹果幼苗超弱发光及一些生理特性的变化[J].西北植物学报,2004,24 (4):720 -724.
[13] 袁佐清.生物超微弱发光研究进展[J].安徽农业科学,2008,36(8):3092- 3094.
[14] 张菊平,张兴志,巩振辉.超微弱发光在蔬菜研究中的应用[J]. 中国农学通报,2006,22(1):220-222.
[15] 李富军, 张新华.微弱发光技术在农产品检测中的应用研究进展[J].农业工程学报,2006,22(12),260-264.
[16] 国家技术监督局.中华人民共和国国家标准:农作物种子检验规程(GB/T3543.3)[M].北京: 中国标准出版社,1995.
[17] 王维光, 顾俭本.从叶片中提取ATP方法的比较[J].植物生理学通讯,1986,(5):54-55.
[18]鲍杰,吴才章. 基于虚拟仪器的小麦活力检测[J].中国粮油学报,2011,26(6):102-105.
[19] 吴才章,徐振方,王继伟.小麦发光特性研究[J].中国粮油学报,2014,29(4):78-81.
[20]郑亚飞.两种蔬菜种子生物超弱发光与活力相关性的研究[J].德州学院学报,2010,26(6):5-7.
[21] 张文兰,田茜,李群,等.低温保存对4 种作物种子超弱发光特性影响规律的研究[J].山东农业科学,2014,46(12):38-42.
[22] 国颖,杨洪强.高温对平邑甜茶幼苗生物发光与能量代谢的影响[J].园艺学报,2008,35(1):99-102.
[23] 林桂玉,黄在范,张翠华,等.菊花花芽分化期超微弱发光及其生理代谢的变化[J].园艺学报,2008,35(12):1819-1824.
[24] 张新华,杨洪强,李富军,等.杏花开放过程中超微弱发光和ATP及活性氧含量的变化[J].植物生理与分子生物学学报,2004,30 (1):41-44.