Abstract: To study the effects of different nitrogen treatments on the content of neutral aroma substances in tobacco leaves after fermentation, a field experiment was carried out in Danzhou, Hainan. The results showed that there was a certain relationship between nitrogen level and the change of the content of neutral aroma substances in Hainan cigar wrapper. With the increase of nitrogen application, the amount of neophytadiene and carotenoids in upper leaves increased, in middle leaves increased first and then decreased. The amount of maillard reaction products in upper leaves showed a tendency of first increase and then decrease; and gradually reduced in middle leaves. The total amount of aromatic amino acid degradation product as a whole showed an increasing trend. The amount of solanone in upper leaves increased first and then decreased; and gradually reduced in middle leaves. Other substances overall presented a decreasing trend. From the content change of the four kinds of aroma substances, nitrogen application T2 (180 kg/hm2) is the best in promoting the amount of cigar wrapper aroma substances.