Abstract: In order to protect the wild resources of Atractylodes chinensis and realize their artificial cultivation，taking the rootand hypocotyl of Atractylodes chinensis as explant，the author studied the callus inducement and differentiation, rooting of adventitious buds. Rooting and transplanting of test-tube seedlings of Atractylodes chinensis, finally established its tissue culture technique system. The results show that the optimum medium for callus inducement and differentiation is 1/2MS (Sugar content is 30 g/L) 6-BA 2 mg/L KT 1 mg/L NAA 0.5 mg/L, 1/2MS (Sugar content is 30 g/L) 6-BA 2 mg/L KT 1 mg/L NAA 0.5 mg/L GA 0.5 mg/L is the optimum medium for rooting.