Current issue

• 2017 Vol.33 No.11 Published:26 April 2017

  Salt Tolerance Screening of Six Maize Varieties Jia Danli,Yang Zhiping,Guo Junling,Guo Caixia and Wang Yongliang 2017, 33 (11):  1-8.  DOI: 10.11924/j.issn.1000-6850.casb16080094 Abstract + ( 1537 )   PDF ( 832KB )   ( 452 ) Screening different varieties of maize salt tolerance, pilot study its physiological mechanisms to explore and exploit maize varieties with excellent tolerance, and lay the foundation for the further study of saline soil field.This experiment uses vermiculite as medium,sets seven NaCl concentration levels of 0mmol/l、45mmol/l、95mmol/l、145mmol/l、195mmol/l、245mmol/l、295mmol/l,tests six maize varieties mass growth at seedling stage of Luyu 39、Huanong 138、Xianyu 335、Aoyu 3007、Yayu 8、Jinping 618, uses the regression equation between growth amount and salt concentration to draw the suitable growth concentration and critical concentration of six maize varieties.Test the activity change of antioxidant enzymes and content change of MDA and proline under different salt concentration stress. Analyze changes of physiological indices under different salt concentration stress. Filter the salt tolerance of six maize varieties.The results showed that the suitable growth NaCl concentration and critical growth NaCl concentration of 6 maize varieties are different. The suitable and critical growth NaCl concentration of Luyu 39 and Huanong 138 are 158.8mmol/l、300mmol/l, which has good salt tolerance. However, the suitable and critical growth NaCl concentration of Jinping 618 is 101.1mmol/l、195.4mmol/l, which has poor salt tolerance.POD activity, SOD activity, CAT activity of six maize varieties leaves, can reach the maximum value at about 195mmol/l salt concentration.With the salt concentration increase, the activity shows increase first then decrease, but it has difference for different varieties increase and decrease value.The content of Proline and MDA were increased with the salt concentration rising, but it varies with the increase value for different varieties. Comprehensive analysis, Luyu 39 and Huanong 138 has better salt tolerance, which is more suitable for further field studies on saline soil.

  Genetic Diversity Analysis of Sugar Beet Resistance to Root Rot by SRAP Markers Wang Maoqian,Zhang Huizhong,Wu Zedong and Wang Huazhong 2017, 33 (11):  9-13.  DOI: 10.11924/j.issn.1000-6850.casb16120038 Abstract + ( 935 )   PDF ( 461KB )   ( 325 ) [Objective][Method]In this study, In order to further analyze the breeding of good varieties of sugar beet resistant to root rot ,10 pairs SRAP molecule markers were used to investigate the relationship analysis of 11 domestic and foreign varieties pairs . [Result] A total of 85 unambiguous bands were obtained,65 of which were polymorphic. The average ratio of polymorphic bands was 76.5%. Compute over-all mean showed that genetic distance was 0.5057, genetic similarity among varieties was 0.6031. The 11 genotypes were divided into 3 cluster groups based on cluster analysis (atintercept of 0.2). [Conclusion]The results showed that the tested varieties of the domestic anti-root rot was better. TY301 (domestic), TY303 (domestic), CK-TY312 (domestic),and HI055 (foreign) anti-root rot were better and the four varieties root rot susceptibility of less than 3%

  A Comparative Study on Rhizosphere Soil Bacterial Communities of Healthy Strawberry and Infected Strawberry with Anthracnose by High-throughput Sequencing Xiao Rong,Cao Qiufen,Nie Yuanjun,Zhang Chunfen,Deng Shu,Sun Haifeng and Li Qian 2017, 33 (11):  14-20.  DOI: 10.11924/j.issn.1000-6850.casb16050118 Abstract + ( 1397 )   PDF ( 623KB )   ( 630 ) The objectives were to compare the diversity of rhizosphere soil bacterial communities of healthy strawberry and infected strawberry with anthracnose, study the bacterial community ecological features involved, and provide evidence for further biocontrol bacteria screening. The diversity analysis was based on the variation of 16S rDNA V3-V4 area and performed by the Illumina Miseq high-throughput sequencing platform. The results showed that the infection resulted in reduced number of operational taxonomic units (OTUs) and diversity index when compared with the healthy specimens. Proteobacteria, bacteroidetes and planctomycetes were dominant shared by the two kind specimens at the phylum level. The two kind specimens had different dominant bacterial compositions at the genus level. In disease soil, the abundance of Pseudomonas was significantly decreased while the abundance of Aeromonas was significantly increased. In addition, the abundance of detoxification bacteria, nitrogen-fixing bacteria, photosynthetic bacteria and nitrifying bacteria in healthy soil were higher than those in disease soil. The rhizosphere soil bacteria abundance and the diversity of healthy strawberry were higher than those of infected strawberry. The structures of bacterial flora were quite different from each other in the two kind specimens. And the relative abundances of beneficial functional microbial groups in healthy soils were higher than that of the disease soils.

  Tissue Culture of Atractylodes chinensis by Orthogonal Design Zuo Jingjing,Yan Guiyun,An Xiaoning,Huo Liguang and Liu Shaoxiang 2017, 33 (11):  21-24.  DOI: 10.11924/j.issn.1000-6850.casb16070023 Abstract + ( 949 )   PDF ( 519KB )   ( 434 ) In order to protect the wild resources of Atractylodes chinensis and realize their artificial cultivation，taking the rootand hypocotyl of Atractylodes chinensis as explant，the author studied the callus inducement and differentiation, rooting of adventitious buds. Rooting and transplanting of test-tube seedlings of Atractylodes chinensis, finally established its tissue culture technique system. The results show that the optimum medium for callus inducement and differentiation is 1/2MS (Sugar content is 30 g/L) 6-BA 2 mg/L KT 1 mg/L NAA 0.5 mg/L, 1/2MS (Sugar content is 30 g/L) 6-BA 2 mg/L KT 1 mg/L NAA 0.5 mg/L GA 0.5 mg/L is the optimum medium for rooting.

  Effects of Potassium Fertilizer Reduction and Split Application on Yield and Quality of Lettuce Fan Xiaowei,Gu Shoukuan,Chen Yi,Yuan Ting,Yang Dong and Wang Zhengyin 2017, 33 (11):  25-34.  DOI: 10.11924/j.issn.1000-6850.casb16070119 Abstract + ( 738 )   PDF ( 556KB )   ( 362 ) The field experiment was conducted to study the effects of different levels potassium fertilizer reduction application by stages on yield and quality of lettuce in purple garden soils. The results showed that the potash fertilizer reduction of 10% - 30% application by stages did not influenced lettuce yields at two experiment sites, the K2 treatment (reduction of K 10% amount of topdressing) had best effects included in the maximum yield (the increase of 4.2% and 4.5%) and the optimum economic profit (84923 yuan/hm2 and 72075 yuan/hm2). Vitamin C content of lettuce leaves showed a downward trend in potassium fertilizer reduction application by stages at two experiment sites, compared with the conventional fertilization (CK) the K2 treatment were significantly decreased vitamin C content of lettuce leaf (8.0% and 8.7%), but the changes of vitamin C content in lettuce stem was different. Potassium fertilizer reduction application by stages reduced nitrate content of lettuce leaves and stems at two experiment sites, the role of K4 treatment (minus the amount of potassium 30% equivalent to topdressing) was the maximum. Considering the balance of sugars in lettuce leaves and stems, K3 treatment was best. Essential amino acid content of lettuce leaves were increased significantly in potassium fertilizer reduction application by stages at two experiment sites but essential amino acid contents of lettuce stem were decreased. Amino acids quality traits of lettuce is the best in K4 and the second was K2. Comprehensive consideration of yield and quality of lettuce, the K2 treatment (reduction 10% potassium fertilizer application by stages) was the best in two purple vegetable soil.

  Effect of Chaetomium Fungal Fertilizer on Growth, Production, Quality of Cucumber and Its Absorption of Nitrogen, Phosphorus and Kalium Zhang Suping,Tian Yehan,Wang Jianming,He Bangling,Li Mingtan and Gao Kexiang 2017, 33 (11):  35-42.  DOI: 10.11924/j.issn.1000-6850.casb16070076 Abstract + ( 1709 )   PDF ( 792KB )   ( 491 ) [Objective]In order to investigate effect of Chaetomium globosum ND35 fungal fertilizer on growth, production, quality of cucumber and absorption of nitrogen, phosphorus, kalium and promote the scientific application of Chaetomium fungal fertilizer, the cucumber ‘Jinchun No.4’ was used as test material. [Method]The experiments of screening application quantity of fungal fertilizer and mixing fungal fertilizer with seaweed fertilizer in cucumber culture were conducted by randomized block arrangement in greenhouse and in the field. [Result]The results showed that the suitable growth quantity of C. globosum ND35 fungal fertilizer was 0.1-0.3 g/plant (3×108 cfu/g). The high amount was detrimental to cucumber growth. The root activity, plant height, stem diameter, leaf area, dry weight, index of seedling quality of cucumber were promoted by using suitable amount of ND35 fungal fertilizer. The photosynthesis efficacy and water use efficiency of cucumber was significantly increased in treatment with ND35 fungal fertilizer and mixed fungal fertilizer with seaweed fertilizer. Total yield of cucumber was increased by 21.85% and 23.90% in application of ND35 fungal fertilizer and mixed treatment. The quality of cucumber was obviously improved by mixed fungal fertilizer with seaweed fertilizer. Compared with control treatment, the content of soluble sugar, soluble protein, vitamin C of cucumber was increased by 7.90%, 172.97% and 301.21% in treatment mixed fungal fertilizer with seaweed fertilizer. The nitrate content decreased 15.57% than that of control. The treatments mixed fungal fertilizer with seaweed fertilizer could promote the absorption of N, P, K in the soil. Specially, absorption of available potassium in the soil was more obvious in the mixed treatment. The N, P, K content of cucumber plant treated with single fungal fertilizer was higher than that of other treatments. Content of P and K in cucumber plant was decreased in treatment mixed fungal fertilizer with seaweed fertilizer. [Conclusion]The experiment result could provide theory evidence for application of Chaetomium fungal fertilizer in good quality and high production of cucumber.

  Leaf Anatomical Comparison of Five Wild Allium Species in Xinjiang Zhu Qingde and Kuerban Sulaiman 2017, 33 (11):  43-47.  DOI: 10.11924/j.issn.1000-6850.casb16050139 Abstract + ( 731 )   PDF ( 2719KB )   ( 281 ) This experiment aims at comparing the leaf anatomy of five species of wild allium plants in Xinjiang and analyzing their environmental adaptability to screen out the wild allium plants suitable for the promotion. [Method] In this experiment, leaves of five species of wild allium plants introduced and cultivated in the experimental base of Xinjiang Agricultural University were chosen as materials to observe their anatomical structures under the optical microscope with paraffin section method. [Result] The results showed that: In specimen NO.1, there were 15~17 large vascular bundles and 45~48 small vascular bundles, while the number of large catheter was 7~10 and that of small catheter was 27~29; In specimen NO.2, there were 5~9 large vascular bundles and 24~26 small vascular bundles, while the number of large catheter was 5~9 and that of small catheter was 8~19; In specimen NO.3, there were 9~11 bundles of large vascular and 19~20 bundles of small vascular, while the number of large catheter was 7~8 and that of small catheter was 3~5; In specimen NO.4, there were 13~14 bundles of large vascular and 20~22 bundles of small vascular, while the quantity of large catheter was 5~10 and that of small catheter was 10~12; In specimen NO.6, there were 8~14 bundles of large vascular and 18~26 bundles of small vascular, while the quantity of large catheter was 6~10 and that of small catheter was 15~20. [Conclusion] It is proved that the specimen NO.1, NO.4 and NO.6 have stronger environmental adaptability than NO.2 and NO.3, and they could be promoted in suitable areas.

  Chromosome Doubling of Potentilla reptans L. var. sericophylla and Its Application Value Zhao Zhenhua and Lin Yinding 2017, 33 (11):  48-53.  DOI: 10.11924/j.issn.1000-6850.casb16070054 Abstract + ( 623 )   PDF ( 623KB )   ( 371 ) In order to obtain higher ornamental value and strong resistance of the variant plants of Potentilla reptans L. var. sericophylla. The change of the multiple of the chromosome group is an important aspect that causes the plant to produce bigger genetic variation and produce new varieties. The normal plant that seeded with colchicine induced double Potentilla chinensis Ser. To compare normal plants, 1 and 2 generations of Potentilla chinensis Ser in plant morphology, pore size, flower size, chlorophyll content, SOD, POD, CAT and character variation analysis. Finally, it was successfully selected and can be stably inherited. There were large leaves, large flower diameter, bright color, high chlorophyll content, high SOD activity, high POD activity and high CAT activity. Have high ornamental and strong resistance of Potentilla chinensis Ser after colchicine treatment. High ornamental and strong resistance of tetraploid can be used as a good groundcover plants applied in garden greening project.

  Pollen viability and stigma receptivity study of Phalaenopsis 2017, 33 (11):  54-58.  DOI: 10.11924/j.issn.1000-6850.casb16060099 Abstract + ( 884 )   PDF ( 4537KB )   ( 371 ) The high activities of pollen and stigma receptivity are the keys to ensure the success of crossbreeding work. To obtain the most applicable time of artificial pollination, scanning electron microscopy and TTC (2,3,5-triphenyltetrazolium chloride) staining were conducted to study the pollen viability of Phalaenopsis ‘Tianxiang’. Meanwhile, the stigma receptivity was determined by the benzidine- H2O2 method. Results indicated that with the anthesis extending, the Phalaenopsis pollinia volume decreased, the color deepened, texture became harder and vitality became weaker; TTC tests showed that the pollen viability rate (dyeing rate) was: open 1 d ＜bud ＜buds unfold period. Stigma tests clarified that when cross-fertilizing within 10-30 d after flowering, success rate of artificial hybridization could be improved, and the rate could reach the maximum within 10-15 d after flowering.

  Effect of Withering Degree on Quality and Catechins Oxidating of Taishun Black Tea Wang Weiwei,Yang Liuyan,Jiang Heyuan and Zhang Jianyong 2017, 33 (11):  59-63.  DOI: 10.11924/j.issn.1000-6850.casb16100017 Abstract + ( 912 )   PDF ( 537KB )   ( 341 ) In order to study the effect of different withering degree on quality and catechin oxidation during Taishun black tea processing, the experiments used the way of natural withering, it set three different treatments named short withering, appropriate withering, long withering by monitoring water content of the withering leaf, it explored the change of tea polyphenols, catechins, theaflavins and theasinesins content during black tea processing. The research showed that the total score of sensory quality was appropriate withering＞short withering＞long withering, short withering treatment got the higher score of liquor colour and infused leaf; tea polyphenols and catechins content of withering leaf decreased with the withering time, and generated theasinesins and a small amount of theaflavins; catechins content decreased, theaflavins and theasinesins content decreased first then ascended during rolling and fermentation processing, comparing with different withering degrees showed that short withering treatment remained and oxidated more catechin and generated more theaflavins, long withering treatment remained and oxidated the least catechin and generated the least theaflavins, the amount of oxidating catechin and generating theaflavins of appropriate withering treatment were between short withering and long withering treatment.

  Selenium Accumulation and Transfer in Soil-Plant System: Influence Factors and Control 2017, 33 (11):  64-68.  DOI: 10.11924/j.issn.1000-6850.casb16060056 Abstract + ( 780 )   PDF ( 497KB )   ( 574 ) Selenium is an important element to animals, plants and microorganisms.Deficient or excessive supply of selenium will cause endemics, such as Keshan disease and large bone disease that may be caused by selenium deficiency.Currently,a large number of scholars conduct comprehensive and deep researches about the effectiveness of selenium in soil, the absorbtion and transportion of selenium in plants as well as its influencing factors.This paper summarizes the influences and the mechanisms of soil texture, pH, organic matter, the redox properties,the plant species, fertilization, soil parent material and the integrated pollution for the migration and transformation of selenium in soil - plant system. This paper describes the problems and deficiencies in current research and proposed the recommendations and advice and outlook for the in-depth study in the future.

  Correlation Analysis of Fruit Quality, Soil Elements and Microorganisms in Navel Orange Orchard Guan Xuefang,Xu Qingxian,Lin Bifen,Qian Lei and Lin Bin 2017, 33 (11):  69-76.  DOI: 10.11924/j.issn.1000-6850.casb16070059 Abstract + ( 634 )   PDF ( 722KB )   ( 430 ) Newhall orange orchard area as the research object, linear correlation and multiple linear regression analysis were used to study the relationship with soil nutrients,microbial communities and navel orange quality. The results showed that: soil organic matter was significantly correlated with reducing sugar and soluble solid content,avail N with the sugar-acid ratio and solid-acid ratio ,Total P with soluble solid content,also avail P with solid-acid ratio;The PLFAs biomarkers 10Me18:0(Actinobacteria),i11:0(Bacteria),16:0(Pseudomonas.sp),17:0

  Effect of Straw Turnover on Soil Nutrients and Soil Microbial Population 2017, 33 (11):  77-80.  DOI: 10.11924/j.issn.1000-6850.casb16050039 Abstract + ( 883 )   PDF ( 446KB )   ( 412 ) In order to inspect Effects of returned-straw on soil nutrients and soil microorganism,we investigated the soil nutrient capacity and soil microorganism amount by field experiment.The results showed that returned-straw could increase the soil organic matter by 0.02-0.04 percent point, increase available nitrogen, available phosphorus, available potassium respectively by 6.64-9.00mg/kg,3.37-4.07mg/kg,10.33-19.00mg/kg,increase amount of soil bateria，fungi and actinomycetes respectively by 2.2×108/g -3.1×108/g、0.5×106/g -3.5×106/g、1.3×107/g-1.9×107/g. But excessive returned-straw amount was not benefit for farmland productivity.

  Effect of Three Chelating Agents on U Polluted Soil Repairing by Sunflower 2017, 33 (11):  81-88.  DOI: 10.11924/j.issn.1000-6850.casb16100097 Abstract + ( 638 )   PDF ( 872KB )   ( 361 ) To study the effect of chelating agent on absorption, accumulation and transport characteristics of sunflower under U contaminated soil is of certain significance. The concentration of U in soil was 150 mg/kg, added EDDS、CA and OA with the concentration of 2.5, 5.0 and 7.5 mmol/kg, and the effects on sunflower growth, U absorption and activation property of U in soil were investigated. The results showed that different treatments of chelating agents decreased the biomass of sunflower, and the decreasing degree reached the largest under high concentration of chelating agent; the addition of CA could increase available U concentration more significantly than EDDS and OA, at the same time, the enrichment coefficient and transport ability of sunflower were also enhanced; the maximum value of the transport coefficient was 0.063 when CA concentration was 7.5 mmol/kg, which was 1.42 times of that of CK. Under this concentration, U content of roots and overground part was 178.95 and 11.27 mg/kg, respectively, which was 2.21 and 3.45 times of that of CK. The maximum activating U content was 104.59 mg/kg, which was 1.63 times of that of CK, U oncentration in roots was higher than that in overground part under all treatments. Therefore, proper addition of chelating agent could increase available U concentration in soil effectively, and promote the absorption and transport of plant.

  Effect of Cd on Plant Growth and Its Tolerance Mechanism Yu Ping,Gao Fan,Liu Jie,Liang Qiong,Han Yingyan,Wang Jingxian and Jia Yuehui 2017, 33 (11):  89-95.  DOI: 10.11924/j.issn.1000-6850.casb16080035 Abstract + ( 1193 )   PDF ( 561KB )   ( 1620 ) Cadmium pollution not only affects plant growth, but also threats to human health, which has been the study hotspot of domestic and foreign. This paper reviewed the effect on Cadmium to plant growth, the mechanism of uptake, transport and resistance of plant to Cadmium. And some new ideas for future research directions are put forward.

  Water Requirement and Precipitation Deficit of Wheat in Yunnan Yang Ke,Wang Pengyu,Wang Hui and Zeng Yan 2017, 33 (11):  96-103.  DOI: 10.11924/j.issn.1000-6850.casb17010101 Abstract + ( 689 )   PDF ( 1809KB )   ( 285 ) Abstract： The paper aims to study the temporal and spatial distribution of natural precipitation deficit in wheat growing period in Yunnan area, and provide technique support for drought disaster prevention and water-saving irrigation. Calling 45-year meteorological data of 115 counties from Yunnan Province from 1972 to 2016, the temporal and spatial distribution characteristics of wheat water requirement and precipitation deficit is analyzed by calculating Penman-Monteith formula.The result indicates that: the inter-annual variability of precipitation of wheat growth period was large and the water requirement is on a rise trend. The average precipitation was 136.4 mm and the average water requirement was 290.9 mm. Years of severe water shortage reached 21% in 87% of these 45 year. At the jointing-booting water requirement key period and heading-blooming stage critical water requirement period, the precipitation deficit rate was high as the wheat was in a severe water shortage. The spatial distribution has shown that the proportion of the 115 wheat planting counties which had a precipitation deficit rate greater than 50% reached 87.1%. Furthermore, the rate of more than 70% accounted for up to 35.3%.

  Comparative Analysis of an Abrupt Rainstorm and a Systemic Rainstorm in Qingyang of Gansu Lu Yaqi,Xu Yan,Wang Yanling and 王燕凌 2017, 33 (11):  104-111.  DOI: 10.11924/j.issn.1000-6850.casb16080088 Abstract + ( 817 )   PDF ( 1996KB )   ( 350 ) Based on the study of the causes and characteristics of an abrupt rainstorm and a systematic rainstorm in Qingyang, Gansu, the authors aim to provide references for the severe rainstorm weather forecast and earlier warning. The conventional meteorological data and diagnostic analysis technology were used to make comparative analysis of the“7.10”abrupt rainstorm and“7.18”systemic rainstorm in Qingyang. The results showed that: (1) the two rainstorms both had low slot at 500 hPa and upper level jet, as well as being upper-cold lower-warm, and the water vapor were from the bay of Bengal, the mesoscale cloud was more active with higher organization degree; (2) the main influencing system of the“7.10”abrupt rainstorm was the shear line, convergence line and the vortex between the two uppers, with a smaller range;“7.18”systemic rainstorm was northeast vortex decline cold air, plateau trough and low-level jet, with a large range and long duration time; (3) the temperature and humidity conditions of“7.10”rainstorm was better, and“7.18”storm water vapor transport channel was more smooth, convergence was more obvious, and ascending motion was more stronger; (4) compared with the“7.18”rainstorm,“7.10”rainstorm had greater instability energy, higher CAPE value and stronger vertical wind shear, but the vertical movement and the convergence divergence were weaker; (5) the“7.10”rainstorm was mainly dominated by isolated convective clouds with very low TBB; “7.18”rainstorm were mainly dominated by mixed cloud with lower TBB.

  Pollution Characteristics of PM2.5 and PM10 and Their Relationships with Meteorological Conditions in Hohhot, 2015 Si Qin,Tong Liga and Zhang Jing 2017, 33 (11):  112-118.  DOI: 10.11924/j.issn.1000-6850.casb16060077 Abstract + ( 921 )   PDF ( 760KB )   ( 480 ) In this paper, pollution characteristics of PM2.5 and PM10 and their relationship with meteorological factors of Hohhot City in 2015 were analyzed using atmospheric environmental monitoring data and meteorological observation data in Hohhot City, and causes of thesevere fog and haze at the end of November in Hohhot City were analyzed preliminarily. The results showed that: (1)fine particulate matter pollution in HohhotCity was heavy, and the pollution of coarse particulate matter was relatively low.The concentrations ofPM2.5 and PM10 were highest in winter and lowest in summer, and the maximum monthly mean value of which in 2015 appeared in December.The trends of PM2.5 and PM10 concentration were basically the same in the whole year, andthe correlation coefficient of annual mean value was 0.711. (2)The correlation coefficient ofconcentrationof PM2.5 and visibility,air pressure,relative humiditywere -0.887, 0.815,0.501,respectively. There is no significant correlation between concentration of PM2.5and wind velocity and direction, precipitation, which may be related to the low annual mean wind velocity, notobviouschange of wind direction and precipitation phase. (3) One of the important reasons of persistent andabnormal strengthening of fog and haze of Hohhot City in November 2015 was related to the long time inversion layer over the Hohhot City, which was caused by increasing humidity (light to moderate drought in spring and summer and precipitation increased significantly in November),weak cold air force, low wind velocity and high temperature phenomenon. And winter heating is another important reason for the strengthening of pollutant concentration in November.

  Control Effect of Bacillus amyloliquefaciens GB58 on Rice Sheath Blight andScreening of Microbial Agent Carrier Lu Yusheng,Gu Wenjie,Jiang Ruiping,Xu Peizhi,Xie Kaizhi and Sun Lili 2017, 33 (11):  119-125.  DOI: 10.11924/j.issn.1000-6850.casb16060029 Abstract + ( 885 )   PDF ( 726KB )   ( 436 ) [Objective] This study aims at researching on the effect of the biocontrol outcome against rice sheath blight disease of the Bacillus amyloliquefaciens GB58 and its most suitable microbial agent carrier to determine the application prospect.[Method] The pot experiment method was adopted to determine the relative lesion height (RLH) of rice sheath blight, colonization ability of rice. The adsorption capacity and stability of GB58 on microbial agent carrier was determined. [Result]The pot experiment result showed that GB58 had an optimistic colonization effect at 2.2×107 cfu/g of the maximum on the diseased plants. Compared to validamycin treatment, the biocontrol effect of GB58 was raised significantly by 20% and 24% respectively in 20d and 40d after inoculating the pathogen. The test on solid microbial agent carrier of GB58 suggested that, taking cell absorption amount, survival rate and antimicrobial activity into consideration, the absorption effect of the organic carrier was better than that of the inorganic. While economic factors were considered comprehensively, the choice of corn meal and organic fertilizer would be ideal.S[Conclusion] Our results came to the conclusion that GB58 had a biocontrol advantage over rice sheath blight disease, while corn meal and organic fertilizer, effective and economical as the microbial agent carrier, had wide application value and great potential.

  Investigation on Weed Occurrence in Highland Barley Field in Lhasa Song Guoying 2017, 33 (11):  126-130.  DOI: 10.11924/j.issn.1000-6850.casb16060052 Abstract + ( 691 )   PDF ( 474KB )   ( 298 ) Induced budding in a greenhouse to soil samples collected from barley field of four counties including13 townships in Lhasa, investigated on weed occurrence in barley field. The results show: there are 12 families, 25 kinds in barley field, mostly annual weed; the main harmful weeds is gramineous weeds, such as Poa annua L., Setaria viridis L., Echinochloa crusgalli L and so on; layered soil cultivation shows: weed emergence amount of 0 to 10 cm soil is higher than 10-20 cm. The study will provide a scientific basis for effective prevention and control weeds in barley field in Lhasa, Tibet.

  Research Progress of Specific Antigen Screening for Taeniasis/Cysticercosis Pang Jianda,Wang Haiying,Du Liyin and Sun Shumin 2017, 33 (11):  131-136.  DOI: 10.11924/j.issn.1000-6850.casb16060088 Abstract + ( 678 )   PDF ( 483KB )   ( 292 ) The study aims to provide theoretical bases for the establishment of immunodiagnosis methods and effective control measures of taeniasis/cysticercosis. We first expounded the prevalence, harm and the problems in the diagnosis, prevention and control of taeniasis/cysticercosis. Then we reviewed the technologies for screening specific antigens of taeniasis/cysticercosis, including cDNA library, phage display library, phage display peptide library and two dimensional PAGE western blotting, briefly described the obtained specific antigen, and expounded potential value of the antigens in diagnosis, prevention and pathogenesis of the disease. Specific antigen screening of taeniasis/cysticercosis provide that it is key to develop the largescale, no leakage antigen screening studies of the disease for solving the problems of immune diagnosis in the different development stages of worm infection and developing vaccine.The study, being important to the diagnosis, prevention and control of taeniasis/cysticercosis, also provides a reference for the diagnosis, prevention and control of other parasitic diseases.

  Remote-sensing Monitoring and Assessment System of Wetland in Northeast China Feng Rui,Sun Longyu,Li Guochun,Ji Ruipeng,Yu Wenying,Wu Jinwen and Zhang Yushu 2017, 33 (11):  137-141.  DOI: 10.11924/j.issn.1000-6850.casb16090088 Abstract + ( 917 )   PDF ( 2145KB )   ( 396 ) Based on the virtual framework structure, the paper established the system of remote sensing monitoring and evaluation for wetlands in Northeast China. Considering the characteristics of the wetland, the system provided basic functions, such as data calibration, projection, geometric correction , resample and so on. The system achieved the calculation of a variety of satellite data and GIS data ,generate various monitoring parameters such as wetland type, wetland area, changes in wetland area and other underlying features of wetlands for its application, realize basic image processing functions such as data registration, data analysis, thematic mapping and image display, give assessment on the influences of climate and cultural features on the change of wetland on the basis of conventional geographic information processing, and publish the remote-sensing monitoring and assessment results and comprehensive assessment with respect to of wetland in Northeast China .

  Variation of Dissolved Oxygen in Water After Passing Through the Ultrasonic Liquid Whistle Aerator Tang Rong,Li Xu,Yao Bangsong,Pei Yi and Xiao Ying 2017, 33 (11):  142-146.  DOI: 10.11924/j.issn.1000-6850.casb16060063 Abstract + ( 898 )   PDF ( 559KB )   ( 509 ) In order to study how water with low dissolved oxygen changes over time after passing liquid whistle ultrasonic aeration equipment, this study conducted static tests of the water after aeration and monitored the changing trends of the dissolved oxygen content in water body over time. In this study, six groups of test were applied and among them, two variables, reed or not and change of flow, were set. The results showed that after the aeration of the equipment in the experimental group, which added a reed, the dissolved oxygen content in the water would continue to increase in the first 2 hours and then reached the maximum value. 2 hours later, the dissolved oxygen content would fall sharply and the descending rate slowed down after 6 hours. When 20 hours passed, the dissolved oxygen content in the test water became stable around 7.4mg/L. The control group reached the peak at 2 hours, and the dissolved oxygen content decreased slowly at 2~3 hours. It would keep increasing later and finally achieved stability around 7.15mg/L after 20 hours. We can get a series of scientific and reasonable variation curve of dissolved oxygen content from the tests and could conclude that adding a reed and smaller flow have a positive influence on aeration. Moreover, this study provides important research value for the aerator application in aquiculture, sewage treatment and oxygen aeration irrigation fields.

  Construction and Sharing Mode of Agricultural Research Information Resources from the Perspective of Big Data Wang Jian and Wu Dingfeng 2017, 33 (11):  147-152.  DOI: 10.11924/j.issn.1000-6850.casb16050140 Abstract + ( 751 )   PDF ( 482KB )   ( 493 ) With deepening of science and technology innovation activities in the field of agriculture, agricultural research information storage, mining, analysis and sharing had faced enormous pressure. Through the deep excavation problems during the process of big data sharing and utilization for agricultural research, this paper combined practical work of sharing and use of agricultural scientific data resources and discussed on the construction and sharing mode of large data resources in agricultural research around the "deposit", "use" and "control" three aspects a global, systematic perspective from global, systematic perspective of big data in agriculture research. Due to large dispersion characteristics of agricultural research information, we designed the big data saving and management institutions structure which changed the traditional secondary data exchange mode of "data unit- data unit" to the third data management mode of “data unit-data storage management mechanism -data unit”. In the structure, we should build some data services platform to provide some kinds of data service, such as data provide services, data analytical services, data mining services, data extension services, and so on. To build data services platform, we could explore the practice mode that included pilot first, step on step, system design discussion, and construction services parallel to ensure data service quality. At the same time, we should focus on institutional building and construct to achieve integration mode which could build orderly top-level cooperation mode of different data platform. Based on these mechanisms that would be built, some construction standards which covered various fields and different themes should be built and all data would be updated dynamically in order to achieve the network information system intercommunication, resource sharing and information exchange. At last, we proposed some advices to enhance sharing and use of large data resources in agricultural research.

  Ecological Carrying Capacity in Reservoir Area of Ankang: Based on Natural Production of Filter-feeding Fish Liu Chao,Li Hansong,Cheng Dingbei,Li Zhi’an,Lv Yajun and Bai Cunjiang 2017, 33 (11):  153-157.  DOI: 10.11924/j.issn.1000-6850.casb16060023 Abstract + ( 575 )   PDF ( 522KB )   ( 382 ) The ecological fish load were calculated under the condition of class II water quality, according to the yield of filter fish in the reservoir area of Ankang and in a proportion of 1 to 3, and to comparison of the calculation results with the phosphorus content in water. Results show, set the cage depth 3m, the unit of water ecological fish carrying capacity is 193.44g/m3, the total output control for 10.47t × 104 in reservoir area of An-kang. When the net raising yield is 100kg/m3, the control area is 1.93 per thousand, and the yield per unit area is inversely proportional to the ecological farming area.

  Planning of Ecological Breeding Park and Case Analysis: Based on Circular Economy Lin Yi,Fan Zengfeng,Huang Xiusheng,Liu Hui and Liu Mingxiang 2017, 33 (11):  158-164.  DOI: 10.11924/j.issn.1000-6850.casb16100006 Abstract + ( 973 )   PDF ( 1086KB )   ( 530 ) Livestock and poultry farming became the major pollution source of COD and NH3-N with the rapid development of animal husbandry. The way about introducing philosophy of agriculture ecological civilization, following principle of circular economy and developing ecological culture park was considered as an effective approach for resolving livestock husbandry pollution at present stage. The study took Fuzhou Genesis Pig Ecological Culture Park as a case study, planned and constructed comprehensive management service center, intensive pig cultivation zone, science and technology culture of circular agriculture zone, circular agriculture ecological farming zone, circular agriculture facility cultivation zone, agricultural products processing and marketing zone around elements of circular economy connotation and followed “3R” principle. Established mode of circular economy of pig ecological breeding based on circular efficient use of meat products and waste (manure) by conducting partition layout reasonably, implementing cleaner production and attaching product ecological design among planting industry, processing industry and leisure tourism industry industrial chain. (1) To deal with pig manure by dry clean up manure method, 70% of pig manure used for gasification power generation; (2) To build biogas pool as the link for flushing water to produce biogas provided fuel energy for entire park, and achieve high biogas utilization by biogas power generation; (3) Biogas slurry used for plating forage grass and then forage grass used for pig, sheep, goose, fish breeding, or used as fertilizer for fruit trees, tea-oil trees and woods directly; (4) To produce high-grade organic fertilizer and protein feed by introducing animal body disposal technology. Expected the scale of amount of 35000 head of the culture park, can produce 11088 kW dry gasification power generation, 2400 kW biogas power generation, 8000 m3 biogas and 1t organic fertilizer per day. This model can reduce organic wastes pollution to the environment, so as to realize recycling cultivation waste multistage circulation. In the protection of the first industry (primary production industry) of high-yield, high-quality, safe, and at the same time, extend the agricultural industrial chain, exploit agricultural market, develop the second industry (industry) and the third industry (leisure tourism industry). Established a new type of agricultural circular economy mode, broaden the agriculture development space, promote agriculture industry from resource consumption type to resource saving and environment friendly type through virtuous cycle of internal substance. Pulled city people stream, logistics, information and science and technology into rural areas, promoted the development of the second and third industry in rural area. In short, the birth of ecological culture park had an important strategic significance for construction of urbanization and a new socialistic countryside, also for integration development of city and countryside.