Research Progress of Specific Antigen Screening for Taeniasis/Cysticercosis

doi:10.11924/j.issn.1000-6850.casb16060088

Abstract

Abstract: The study aims to provide theoretical bases for the establishment of immunodiagnosis methods and effective control measures of taeniasis/cysticercosis. We first expounded the prevalence, harm and the problems in the diagnosis, prevention and control of taeniasis/cysticercosis. Then we reviewed the technologies for screening specific antigens of taeniasis/cysticercosis, including cDNA library, phage display library, phage display peptide library and two dimensional PAGE western blotting, briefly described the obtained specific antigen, and expounded potential value of the antigens in diagnosis, prevention and pathogenesis of the disease. Specific antigen screening of taeniasis/cysticercosis provide that it is key to develop the largescale, no leakage antigen screening studies of the disease for solving the problems of immune diagnosis in the different development stages of worm infection and developing vaccine.The study, being important to the diagnosis, prevention and control of taeniasis/cysticercosis, also provides a reference for the diagnosis, prevention and control of other parasitic diseases.

Pang Jianda,Wang Haiying,Du Liyin and Sun Shumin. Research Progress of Specific Antigen Screening for Taeniasis/Cysticercosis[J]. Chinese Agricultural Science Bulletin, 2017, 33(11): 131-136.

References

[1] Verster A. Redescription of Taenia solium Linnaeus, 1758 and Taenia saginata Goeze, 1782[J].Z Parasitenkd,1967,29(4):313-328.
[2] Wu HW, Ito A, Ai L, et al. Cysticercosis/taeniasis endemicity in Southeast Asia: Current status and control measures[J].Acta Trop,2016:S0001-706X(16)30013-4.
[3] WHO. Working to overcome the global impact of neglected tropical diseases-Summary[J].Wkly Epidemiol Rec,2011,86:113-120.
[4] FAO. Identifies top 10 foodborne parasites[J].Vet Rec,2014,175(3):58.
[5] Krecek R C, Mohammed H, Michael LM, et al. Risk factors of porcine cysticercosis in the Eastern Cape Province, South Africa[J].PLoS One,2012,7(5):e37718.
[6] Conlan J V, Sripa B, Attwood S, et al. A review of parasitic zoonoses in a changing Southeast Asia[J].Vet Parasitol,2011,182(1):22-40.
[7] Wandra T, Swastika K, Dharmawan NS, et al. Thepresent situation and towards the prevention and control of neurocysticercosis onthe tropical island, Bali, Indonesia. Parasit Vectors. 2015,8:148.
[8] Wu W, Qian X, Huang Y, et al. Review of the control of Clonorchiasis sinensis and Taenia solium taeniasis/cysticercosis in China[J].Parasitol Res,2012,111(5):1879-1884.
[9] 刘明远,刘全,方维焕,等.我国的食源性寄生虫病及其相关研究进展[J].中国兽医学报,2014(7):1205-1224.
[10] Xu, A, Gu, J. The current situation and the prevalenct trend of cysticercosis in China[J].China Tro Med,2010,10:239-240.
[11] Wu, W, Jia, F, Wang, W. et al. Antiparasitic treatment of cerebral cysticercosis: lessons and experiences from China[J].Parasitol Res,2013,112:2879-2890.
[12] Bianchin M M, Velasco T R, Coimbra E R, et al. Cognitive and surgical outcome in mesial temporal lobe epilepsy associated with hippocampal sclerosis plus neurocysticercosis: a cohort study[J].PLoS One,2013,8(4):e60949.
[13] Sako Y, Nakao M, Nakaya K, et al. Recombinant antigens for serodiagnosis of cysticercosis and echinococcosis[J].Parasitol Int,2006,55:S69-S73.
[14] Lightowlers M W. Eradication of Taenia solium cysticercosis: a role for vaccination of pigs[J].Int J Parasitol,2010,40(10):1183-1192.
[15] Mkupasi E M, Sikasunge C S, Ngowi H A, et al. Detection of cysteine protease in Taenia solium-induced brain granulomas in naturally infected pigs[J].Vet Parasitol,2013,197(1-2):360-363.
[16] Gauci C G, Jayashi C M, Gonzalez A E, et al. Protection of pigs against Taenia solium cysticercosis by immunization with novel recombinant antigens[J].Vaccine,2012,6,30(26):3824-3828.
[17] Jayashi CM, Gonzalez AE, Castillo Neyra R, et al. Characterisation of antibody responses in pigs induced by recombinant oncosphere antigens from Taenia solium[J].Vaccine,2012,14,30(52):7475-7480.
[18] Atluri S R, Singhi P, Khandelwal N, et al. Evaluation of excretory secretory and 10-30 kDa antigens of Taenia solium Cysticerci by EITB assay for the diagnosis of neurocysticercosis[J]. Parasite Immunol,2009,31(3):151-155.
[19] Noh J, Rodriguez S, Lee YM, et al. A recombinant protein and synthetic peptide-based immunoblot test for diagnosis of neurocysticercosis[J].J Clin Microbiol,2014,19.
[20] Ferrer E, Sánchez J, Milano A, et al. Diagnostic epitope variability within Taenia solium 8 kD antigen family: implications for cysticercosis immunodetection[J]. Exp Parasitol,2012,130(1):78-85.
[21] Salazar-Anton F, Lindh J. Taenia solium: a two-dimensional Western blotting method combined with the use of an EST-library for the identification of immunogenic proteins recognized by sera from neurocysticercosis patients[J].Exp Parasitol,2011,128(4):371-376.
[22] Mwape KE, Praet N, Benitez-Ortiz W,et al. Field evaluation of urine antigen detection for diagnosis of Taenia solium cysticercosis[J].Trans R Soc Trop Med Hyg,2011,105(10):574-578.
[23] Hofstetter H, Schamb?ck A, Van Den Berg J, et al. Specific excision of he inserted DNA segment from hybrid plasmids constructed by the poly(dA). poly(dT) method[J]. Biochim Biophys Acta,1976,454(3):587-591.
[24] Johnson K S, Harrison G B, Lightowlers M W, et al. Vaccination against ovine cysticercosis using a defined recombinant antigen[J].Nature,1989,338(6216):585-587.
[25] Landa A, Laclette J P, Nicholson Weller A, et al. cDNA cloning and recombinant expression of collagen-binding and complement inhibitor activity of Taenia solium paramyosin(AgB)[J].Molecular and Biochemical parasitology,1993,60(2):343-347.
[26] Montero E, González L M, Harrison L J, et al. Taenia solium cDNA sequence encoding a putative immunodiagnostic antigen for human cysticercosis[J].J Chromatogr B Analyt Technol Biomed Life Sci,2003,786(1-2):255-269.
[27] 孙树汉,王俊霞,陈蕊雯,等.编码猪囊尾蜘病的免疫诊断抗原cDNA分子克隆[J].中国寄生虫学与寄生虫病杂志,1997,15(1):15-20.
[28] 沈斌,高荣,朱锦,等.猪囊尾蝴cDNA表达文库构建及抗原基因的筛选[J].中国人兽共患病杂志,1999,15(6):57-59.
[29] 张莉,史宇晖,刘殿武,等.猪囊尾蝴cDNA文库的构建[J].中国寄生虫病防治,2000,13(3):190-193.
[30] 翟春生,唐雨德,顾志香.猪带绦虫六钩蝴抗原基因的克隆与表达[J].中国兽医学报,1997,17(5):470-472.
[31] 唐雨德,陆承平.猪带绦虫六钩蚴cDNA文库的构建与筛选[J].中国兽医学报,2003,23(6):567-569.
[32] 景志忠,郭爱疆,海岗,等.猪带绦虫六钩蚴cDNA文库的构建及免疫原基因的筛选与克隆[J].畜牧兽医学报,2005,6(4):391-396.
[33] 黄江,胡旭初,徐劲,等.猪带绦虫成虫cDNA质粒文库的构建及EST测序[J].中国人兽共患病学报,2008,24(12):1126-1128.
[34] Smith G P. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface[J].Science,1985,228(4705):1315-1317.
[35] Robles Y, González E, Govezensky T, et al. Isolation of the Taenia crassiceps antigens from a phage display cDNA library and evaluation of their use for diagnosis of neurocysticercosis[J].Clin Immunol,2005,116(3):265-270.
[36] González E, Robles Y, Govezensky T, et al. Isolation of neurocysticercosis-related antigens from a genomic phage display library of Taenia solium[J].J Biomol Screen,2010,15(10):1268-1273.
[37] Dela Cruz V F, Lal A A, Mc Cutchan T F. Immunogenicity and epitope mapping of foreign sequences via genetically engineered filamentous phage[J].J Biol Chem,1988,263:4318-4322.
[38] 李天群,雷家慧,姜昌富.囊虫抗原模拟表位的筛选和序列分析[J].中国人兽共患病杂志,2004,20(11):968-970.
[39] Manoutcharian K, Sotelo J, Garcia E, et al. Characterization of cerebrospinal fluid antibody specificities in neurocysticercosis using phage display peptide library[J].Clin Immunol,1999,91(1):117-121.
[40] Gazarian K G, Solis C F, Gazarian T G, et al Synthetic peptide-targeted selection of phage display mimotopes highlights immunogenic features of α-helical vs non-helical epitopes of Taenia solium paramyosin: implications for parasite and host-protective roles of the protein[J].Peptides,2012,34(1):232-241.
[41] Hell R C, Amim P, de Andrade H M, et al Immunodiagnosis of human neurocysticercosis using a synthetic peptide selected by phage-display[J].Clin Immunol,2009,131(1):129-138.
[42] Rassy D, Bobes R J, Rosas G, et al Characterization of S3Pvac anti-cysticercosis vaccine components: implications for the development of an anti-cestodiasis vaccine[J].PLoS One,2010,5(6):e11287.
[43] O''Farrell P H. High resolution two-dimensional electrophoresis of proteins[J].J Biol Chem,1975,250(10):4007-4021.
[44] 王丽娜,葛凌云,董彩华,等.双向电泳及免疫印记法对囊尾蚴囊液抗原的分析、纯化及鉴定[J].中国热带医学,2003,3(6):717-719.
[45] 方文,包怀恩,肖靓靓,等.猪囊尾蚴特异性抗原的筛选、鉴定和生物信息学分析[J].中国寄生虫学与寄生虫病杂志,2009,27(4):313-317.
[46] 方文,肖靓靓,包怀恩,等.猪带绦虫囊尾蚴与亚洲带绦虫囊尾蚴蛋白双向电泳图谱分析[J].中国寄生虫学与寄生虫病杂志,2011,29(3):188-190.
[47] Mayta H, Hancock K, Levine M Z, et al. Cysticercosis Working Group in Peru. Characterization of a novel Taenia solium oncosphere antigen[J].Mol Biochem Parasitol,2007(2):154-161.
[48] Levine M Z, Calderón J C, Wilkins P P, et al .Characterization, cloning, and expression of two diagnostic antigens for Taenia solium tapeworm infection[J].J Parasitol,2004,90(3):631-638.