Genetic Diversity Analysis of Sugar Beet Resistance to Root Rot by SRAP Markers

doi:10.11924/j.issn.1000-6850.casb16120038

Abstract

Abstract: [Objective][Method]In this study, In order to further analyze the breeding of good varieties of sugar beet resistant to root rot ,10 pairs SRAP molecule markers were used to investigate the relationship analysis of 11 domestic and foreign varieties pairs . [Result] A total of 85 unambiguous bands were obtained,65 of which were polymorphic. The average ratio of polymorphic bands was 76.5%. Compute over-all mean showed that genetic distance was 0.5057, genetic similarity among varieties was 0.6031. The 11 genotypes were divided into 3 cluster groups based on cluster analysis (atintercept of 0.2). [Conclusion]The results showed that the tested varieties of the domestic anti-root rot was better. TY301 (domestic), TY303 (domestic), CK-TY312 (domestic),and HI055 (foreign) anti-root rot were better and the four varieties root rot susceptibility of less than 3%

CLC Number:

S566.3

Wang Maoqian,Zhang Huizhong,Wu Zedong and Wang Huazhong. Genetic Diversity Analysis of Sugar Beet Resistance to Root Rot by SRAP Markers[J]. Chinese Agricultural Science Bulletin, 2017, 33(11): 9-13.

References

[1] 乔志文.甜菜根腐病综合防治技术研究[D].北京:中国农业科学院,2007.
[2] 咸洪泉,刘杰贤.甜菜根腐病综合防治技术探讨[J].中国甜菜糖业,1998(2):29-32.
[3] 王大萍,徐丹丹,何鑫,等.中国甜菜根腐病研究进展[J].生物技术世界,2012(6):56,58-58.
[4] 乔志文,张杰,赵海英.国外甜菜褐斑病、根腐病、黄化病毒病研究进展[J].中国甜菜,2011,(3):55-59.
[5] 胡英凯.甜菜根腐病的发生与防治[J].农林科学实验,2013(22):121-121,134.
[6] 倪洪涛,倪洪波,张福顺.分子标记技术在甜菜育种中的应用[J].中国农学通报,2016,32(16):132-137.
[7] 吴则东,张文彬,王华忠,等.甜菜遗传与育种研究进展[J].中国甜菜,2014(2):71-73,76.
[8] 王华忠,吴则东,王晓武,等.利用SRAP与SSR标记分析不同类型甜菜的遗传多样性[J].作物学报,2008,34(1):37-46.
[9] 王华忠.甜菜全DNA提取方法及RFLP分析技术[J].中国糖料,1997(4):44-47.
[10] 代培红,腊萍,郭长奎,等.甜菜SSR反应体系优化及重要农艺性状分子标记[J].新疆农业科学,2013,50(7):1199-1205.
[11] 吴则东,王茂芊,马龙彪,等.适于甜菜品种鉴定的SSR核心引物的筛选[J].中国农学通报,2015,31(15):165-169.
[12] 牛泽如,杨文柱,庞磊,等.基于ISSR和AFLP标记开发甜菜SSR引物[J].中国农学通报,2010,26(21):147-151.
[13] 腊萍,罗淑萍,章建新,等.甜菜RAPD反应体系优化及亲缘关系研究[J].新疆农业大学学报,2010,33(1):1-6.
[14] Li G, Quiros C F. Sequence-related amplified polymorphism(SRAP),A new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brossica[J].Theoretical and Applied Genetics,2001,103:455-461.
[15] Budak H, Shearman R C, Parmaksiz I, et al. Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers[J]. Theor. Appl. Genet.,2004,108:328-334.
[16] 陈昆松,李方,徐昌杰,等.改良CTAB法用于多年生植物组织基因组DNA的大量提取[J].遗传,2004,26(4):529-531.
[17] 王茂芊,张立明,王维成,等.西北地区甜菜品系遗传多样性的SRAP分析[J].中国农学通报,2011,27(22):156-161.
[18] 王茂芊,白晨,吴则东,等.国内外18个甜菜品种的根腐病和褐斑病抗性鉴定[J].中国糖料,2015(4):8-10.
[19] 吴旭红.甜菜抗根腐病基因ISSR分子标记的初步研究[J].齐齐哈尔大学学报:自然科学版,2005,21(2):30-32.
[20] 周春娥,王芳,周延清,等.大豆遗传多样性的SRAP分析[J].江苏农业科学,2012,40(1):40-42.
[21] 杨在君,彭正松,周永红,等.利用SRAP分子标记评价小麦三雌蕊近等基因系的遗传背景[J].核农学报,2012,26(1):22-27.
[22] Ferriol M, Pico B, Nuez F. Genetic diversity of a germplasm collection of cucurbita pepo using SRAP and AFLP markers[ J]. Theor Appl Genet, 2003,107:271-282
[23] 伊六喜,斯钦巴特尔,孟显光,等.甜菜雄性核不育基因的SRAP标记[J].华北农学报,2012(1):106-109.
[24] 王茂芊,吴则东,王华忠.利用SRAP标记分析中国甜菜三大产区骨干材料的遗传多样性[J].作物学报,2011,37(5):811-819.
[25] 吴则东,王凤华,喻丹丹,等.适合甜菜品种鉴定的SRAP核心引物的筛选[J].中国农学通报,2015,31(30):234-238.