Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2022, Vol. 38 ›› Issue (21): 17-23.doi: 10.11924/j.issn.1000-6850.casb2021-1173

Special Issue: 生物技术 园艺

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Drought Resistance of Sugar Beet Seedling: Identification and Index Screening

LI Wangsheng1,2(), WANG Xueqian1,2, YIN Xilong1,2, SHI Yang1,2, XING Wang1,2()   

  1. 1National Beet Germplasm Mid-term Bank, Heilongjiang University, Harbin 150080
    2Key Laboratory of Beet Genetics and Breeding, College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
  • Received:2021-11-20 Revised:2022-03-28 Online:2022-07-25 Published:2022-08-23
  • Contact: XING Wang E-mail:1595764058@qq.com;xyjiayou_086@163.com

Abstract:

To screen the drought-resistant sugar beet germplasm resources at the seedling stage and determine the drought-resistant indexes, in this study, 24 beet germplasms provided by the National Beet Germplasm Bank were used as materials, PEG-6000 was used to simulate drought stress, and Hoagland hydroponic solution (CK) under normal growth condition was used as control. Sugar beet seedlings were treated with 6% PEG-6000 for 7 days, and the phenotype indexes of aboveground and underground parts were determined. The results showed that the effects of drought stress on leaf fresh weight, root fresh weight, leaf saturated fresh weight and leaf dry weight were significant, with a decrease rate of more than 50%, and the root-to-shoot ratio of sugar beet germplasm increased significantly after drought stress. Cluster analysis divided the 24 beet germplasms into four categories, of which, ZT000516 belonged to one category alone, which was the most drought-resistant germplasm resource. The second category had strong drought-resistance, including ZT000078, ZT000547 and ZT000247. The third category was medium drought-resistant, which had a total of 7 germplasms. The fourth category was low drought-resistant, which had 13 germplasms, of which, ZT001698 and ZT001398 were highly drought-sensitive germplasms. By stepwise regression analysis, leaf dry weight and leaf saturated fresh weight were significantly correlated with D value. ZT000516 was a germplasm with strong drought resistance, and ZT001698 and ZT001398 were drought-sensitive germplasms. Leaf dry weight and leaf saturated fresh weight could be used as indexes for rapid and accurate identification of drought stress in sugar beet seedling stage.

Key words: sugar beet, seedling stage, drought stress, identification of drought resistance, cluster analysis

CLC Number: