Methanol Aging Treatment: Effect on Germination of Sugar Beet Seeds

doi:10.11924/j.issn.1000-6850.casb2022-0023

Abstract

Abstract:

The paper aims to study the effect of artificial aging treatment with methanol solution on the germination index of sugar beet seeds, explore the storage resistance and aging mechanism of sugar beet seeds, and provide a theoretical basis for understanding the artificial aging of sugar beet seeds. In this study, TX2020, a newly harvested single seed, was used as experimental material, and 6 aging time (5, 10, 15, 20, 25 and 30 min) were set up. The seeds were treated with methanol aging method, and the germination test was carried out on the seeds. The germination potential, germination rate, germination index, vigor index, total length of radicle and hypocotyl and conductivity of seed extract were determined. The results showed that germination indexes of seeds decreased after aging treatment. Compared with those of the control, the germination potential, germination rate, germination index, vigor index and total length of radicle and hypocotyl were decreased by 8.8%-31.41%, 13.96%-34.23%, 2.66%-22.44%, 16.63%-36.44% and 7.54%-23.06%, respectively, while the conductivity of seed extract was increased compared with that of the control by 34.73%-76.19%. The results showed that methanol solution could inhibit the germination of sugar beet seeds. With different aging time, the inhibition effect of methanol solution on the germination indexes of sugar beet seeds was also different.

Key words: sugar beet, methanol, seed aging, germination index

CLC Number:

S566.3

LIU Na, HU Huabing, WANG Ronghua, LIU Xiaoyue, LIU Zhaoyang, LIU Xiaohan, WANG Maoqian. Methanol Aging Treatment: Effect on Germination of Sugar Beet Seeds[J]. Chinese Agricultural Science Bulletin, 2022, 38(33): 28-33.

Figures/Tables 8

References 25

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处理编号	甲醇浓度	老化时间/min
处理1	50%甲醇溶液	5
处理2	50%甲醇溶液	10
处理3	50%甲醇溶液	15
处理4	50%甲醇溶液	20
处理5	50%甲醇溶液	25
处理6	50%甲醇溶液	30

处理编号	甲醇浓度	老化时间/min
处理1	50%甲醇溶液	5
处理2	50%甲醇溶液	10
处理3	50%甲醇溶液	15
处理4	50%甲醇溶液	20
处理5	50%甲醇溶液	25
处理6	50%甲醇溶液	30

处理编号	发芽势/%	发芽率/%	发芽指数	活力指数	胚根胚轴总长/cm
1	58.00±6.00 abc	63.67±6.66 ab	17.39±1.59 a	122.70±20.46 c	7.04±0.69 d
2	51.33±12.06 bc	52.67±13.58 bc	17.70±4.85 a	143.34±42.94 c	8.08±0.47 c
3	54.00±2.65 bc	55.33±3.21 bc	17.00±1.24 a	143.89±12.97 c	8.46±0.29 bc
4	62.00±4.58 ab	63.33±4.04 ab	21.08±0.40 a	160.95±3.24 bc	7.63±0.27 cd
5	46.67±5.51 c	50.33±3.79 bc	16.35±0.90 a	136.86±9.80 c	8.37±0.22 bc
6	47.33±9.07 c	48.67±10.26 c	20.52±3.61 a	218.26±27.32 a	10.71±0.68 a
CK	68.00±3.61 a	74.00±3.61 a	21.08±1.25 a	193.06±19.69 ab	9.15±0.47 b

处理编号	发芽势/%	发芽率/%	发芽指数	活力指数	胚根胚轴总长/cm
1	58.00±6.00 abc	63.67±6.66 ab	17.39±1.59 a	122.70±20.46 c	7.04±0.69 d
2	51.33±12.06 bc	52.67±13.58 bc	17.70±4.85 a	143.34±42.94 c	8.08±0.47 c
3	54.00±2.65 bc	55.33±3.21 bc	17.00±1.24 a	143.89±12.97 c	8.46±0.29 bc
4	62.00±4.58 ab	63.33±4.04 ab	21.08±0.40 a	160.95±3.24 bc	7.63±0.27 cd
5	46.67±5.51 c	50.33±3.79 bc	16.35±0.90 a	136.86±9.80 c	8.37±0.22 bc
6	47.33±9.07 c	48.67±10.26 c	20.52±3.61 a	218.26±27.32 a	10.71±0.68 a
CK	68.00±3.61 a	74.00±3.61 a	21.08±1.25 a	193.06±19.69 ab	9.15±0.47 b