Pathogenic Bacteria of Sugar Beet Blight: Isolation and Identification

doi:10.11924/j.issn.1000-6850.casb2021-1177

Abstract

Abstract:

The study aims to identify the pathogenic bacterial species responsible for sugar beet blight on the Hulan campus of Heilongjiang University and provide theoretical reference for the scientific prevention and treatment of the disease. Sugar beet blight plants collected from the Hulan campus of Heilongjiang University were used as test materials, and the pathogens were isolated and characterized by morphological observation, pathogenicity determination, and molecular biology according to the Koch's law. The results showed that strain L1 isolated from the roots of pathogenesis sugar beet seedlings had white hyphae. Large conidia were of the sickle type and had septa, and small conidia were mostly single cell with a few septa. The molecular identification results showed that this pathogen was in the same clade at the Fusarium saprophyticus group on a phylogenetic tree constructed based on ITS and tef1 sequences. Based on the morphological and molecular characterization results, the pathogen responsible for causing sugar beet blight on the Hulan campus of Heilongjiang University was determined to be Fusarium solani.

Key words: sugar beet blight, pathogenic bacteria, Fusarium, Fusarium solani, isolation and identification

CLC Number:

S566

LIU Danyang, CUI Rufei, GENG Gui, WANG Yuguang. Pathogenic Bacteria of Sugar Beet Blight: Isolation and Identification[J]. Chinese Agricultural Science Bulletin, 2022, 38(24): 113-117.

Figures/Tables 6

References 28

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