Research Progress on Endophytes from Glycyrrhiza uralensis

doi:10.11924/j.issn.1000-6850.casb2021-0789

Abstract

Abstract:

Endophytes isolated from Glycyrrhiza uralensis have the potential to produce the same, similar or new active metabolites as the host plants. Secondary metabolites of Glycyrrhiza uralensis produced by fermentation with endophytes are not only important for sustainable utilization of Glycyrrhiza uralensis resources but also significant for screening new metabolites. In the past several decades, although the diversity, isolation and identification, pharmacological action, secondary metabolites and other aspects of endophytes from Glycyrrhiza uralensis have been studied and some achievements have been obtained, it is far from meeting the needs of production. As a microbial resource, endophytes from Glycyrrhiza uralensis are an important source of natural products. The study reviewed the research on endophytes from Glycyrrhiza uralensis and their metabolites, and summarized that endophytes from Glycyrrhiza uralensis could produce liquiritin, glycyrrhetinic acid, liquiritigenin and other secondary metabolites. Meanwhile, according to the problems in the research on secondary metabolites of endophytes from Glycyrrhiza uralensis, the study discussed the prospect and provided certain reference for the rational utilization of endophytes from Glycyrrhiza uralensis.

Key words: endophytes from Glycyrrhiza uralensis, diversity, isolation and identification, pharmacological action, secondary metabolites

CLC Number:

R931

YOU Mengyao, WAN Lu, YAN Jiajia, ZHANG He, ZHENG Chunying. Research Progress on Endophytes from Glycyrrhiza uralensis[J]. Chinese Agricultural Science Bulletin, 2022, 38(26): 20-26.

Figures/Tables 4

References 55

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序号	产地	基原	消毒方法	内生真菌/株	内生细菌/株	文献来源
1	新疆	胀果甘草根	（1）70%酒精浸5 s,0.1% HgCl₂浸5 min, 再用无菌水冲洗5遍。（2）70%酒精浸5 s,5% NaClO₄浸5 min, 再用无菌水冲洗5遍。	2（为青霉菌属和镰刀菌属）	149	宋素琴等^[34]
2	内蒙古鄂尔多斯市	甘草根部	75%乙醇浸泡5~10 min表面消毒,然后用无菌水冲洗5 min去除表面消剂, 再用0.525% NaOCl浸泡7 min,无菌水冲洗5次,无菌滤纸吸干水分。	1（为镰孢霉属）	0	王红霞等^[35]
3	黑龙江省大庆地区	乌拉尔甘草叶	75%酒精漂1 min,10%双氧水15 min,7 5%酒精漂洗1 min,无菌水冲洗4次。	0	32	姜硕等^[36]
4	宁夏盐池	甘草根茎叶	3%次氯酸钠浸泡1~3 min,无菌水冲洗5次, 0.1%氯化汞浸泡1~3 min, 无菌水冲洗5次;75%乙醇浸泡1~3 min; 无菌水冲洗5次。	0	100	郎多勇等^[37]
5	甘肃省酒泉市金塔县	乌拉尔甘草	无菌水冲洗5次;75%乙醇浸泡3 min; 无菌水冲洗5次;0.1%升汞浸泡3 min; 无菌水冲洗5次。	94	64	杨志军等^[38]
6	黑龙江省大庆地区	乌拉尔甘草根茎	用75%的酒精浸泡1 min,放在2% NaClO 浸泡15 min,无菌水冲洗3次。	46	0	吴桐^[39]

序号	产地	基原	消毒方法	内生真菌/株	内生细菌/株	文献来源
1	新疆	胀果甘草根	（1）70%酒精浸5 s,0.1% HgCl₂浸5 min, 再用无菌水冲洗5遍。（2）70%酒精浸5 s,5% NaClO₄浸5 min, 再用无菌水冲洗5遍。	2（为青霉菌属和镰刀菌属）	149	宋素琴等^[34]
2	内蒙古鄂尔多斯市	甘草根部	75%乙醇浸泡5~10 min表面消毒,然后用无菌水冲洗5 min去除表面消剂, 再用0.525% NaOCl浸泡7 min,无菌水冲洗5次,无菌滤纸吸干水分。	1（为镰孢霉属）	0	王红霞等^[35]
3	黑龙江省大庆地区	乌拉尔甘草叶	75%酒精漂1 min,10%双氧水15 min,7 5%酒精漂洗1 min,无菌水冲洗4次。	0	32	姜硕等^[36]
4	宁夏盐池	甘草根茎叶	3%次氯酸钠浸泡1~3 min,无菌水冲洗5次, 0.1%氯化汞浸泡1~3 min, 无菌水冲洗5次;75%乙醇浸泡1~3 min; 无菌水冲洗5次。	0	100	郎多勇等^[37]
5	甘肃省酒泉市金塔县	乌拉尔甘草	无菌水冲洗5次;75%乙醇浸泡3 min; 无菌水冲洗5次;0.1%升汞浸泡3 min; 无菌水冲洗5次。	94	64	杨志军等^[38]
6	黑龙江省大庆地区	乌拉尔甘草根茎	用75%的酒精浸泡1 min,放在2% NaClO 浸泡15 min,无菌水冲洗3次。	46	0	吴桐^[39]

序号	产地	基原	菌种	分离得到活性化合物个数/个	参考文献
1	黑龙江省大庆地区	乌拉尔甘草	内生细菌 GYX9发酵液	1	姜硕等^[36]
2	黑龙江省大庆地区	乌拉尔甘草根部	内生真菌 HGC6发酵液	4	郑雪等^[48]
3	黑龙江省大庆地区	乌拉尔甘草	内生真菌 GJZ2发酵液	1	孟利等^[49]
4	黑龙江省帽儿山地区	乌拉尔甘草	内生真菌 HGE5发酵液	1	李伟等^[50]
5	黑龙江省帽儿山	乌拉尔甘草	内生真菌 RE7 发酵液	1	徐慧超等^[51]
6	黑龙江省大庆地区	乌拉尔甘草叶	内生真菌 GYP8发酵液	1	姜硕等^[52]
7	黑龙江省大庆地区	乌拉尔甘草叶	内生真菌 GRE9发酵液	13（挥发性成分）	许哲祥等^[53]

序号	产地	基原	菌种	分离得到活性化合物个数/个	参考文献
1	黑龙江省大庆地区	乌拉尔甘草	内生细菌 GYX9发酵液	1	姜硕等^[36]
2	黑龙江省大庆地区	乌拉尔甘草根部	内生真菌 HGC6发酵液	4	郑雪等^[48]
3	黑龙江省大庆地区	乌拉尔甘草	内生真菌 GJZ2发酵液	1	孟利等^[49]
4	黑龙江省帽儿山地区	乌拉尔甘草	内生真菌 HGE5发酵液	1	李伟等^[50]
5	黑龙江省帽儿山	乌拉尔甘草	内生真菌 RE7 发酵液	1	徐慧超等^[51]
6	黑龙江省大庆地区	乌拉尔甘草叶	内生真菌 GYP8发酵液	1	姜硕等^[52]
7	黑龙江省大庆地区	乌拉尔甘草叶	内生真菌 GRE9发酵液	13（挥发性成分）	许哲祥等^[53]