Amomum tsao-ko in Yunnan Province: Identification of Major Pathogenic Fungi and Screening of Control Fungicides

doi:10.11924/j.issn.1000-6850.casb2021-0369

Abstract

Abstract:

In order to identify the fungal pathogens causing the main diseases of Amomum tsao-ko in Yunnan, screen the fungicides which can effectively inhibit the pathogens, and provide a basis for chemical control of Amomum tsao-ko diseases, tissue separation and purification culture were conducted, and the classification status of pathogens were identified and analyzed with morphology identification and ITS, TEF, ACT and GAPDH sequence analysis. The control effects of 7 fungicides on Amomum tsao-ko diseases were studied by using mycelial growth rate method. The results showed that the isolated strains were Fusarium graminearum and Colletotrichum gloeosporioides. Screening results of control agents showed that the inhibition rate of the F. graminearum reached 93% by Jingcai. Within the recommended range in field, the inhibition rate of the C. gloeosporioides reached 100% by Mancozeb. Lankai, Jingcai and Shihuile all achieved more than 90% control effect at low concentration. Jingcai, Shihuile, Mancozeb and Lankai are recommended as the optimal fungicides for Amomum tsao-ko fungal diseases in Yunnan Province.

Key words: Amomum tsao-ko diseases, isolation and identification, pathogenicity, control efficiency, mycelial growth rate method

CLC Number:

S453.621

WU Jiajia, ZHU Jiahong, CAO Aocheng, YAN Dongdong, WANG Qiuxia, ZHANG Chunhua, LI Yuan. Amomum tsao-ko in Yunnan Province: Identification of Major Pathogenic Fungi and Screening of Control Fungicides[J]. Chinese Agricultural Science Bulletin, 2022, 38(6): 121-128.

Figures/Tables 10

References 30

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杀菌剂	剂型	推荐剂量	生产厂家
40%异菌脲	WP	1125~1500 g/hm²	富美实（中国)投资有限公司
80%代森锰锌	WP	3000~3750 g/hm²	青岛东生药业有限公司
250 g/L嘧菌酯	SC	720~900 mg/hm²	先正达（南通）公司
38%唑醚·啶酰菌（蓝楷）	SC	450~600 mg/hm²	河北中保绿农作物科技有限公司
32.5%苯甲·嘧菌酯（京彩）	SC	450~750 g/hm²	河北中保绿农作物科技有限公司
32.6%苯甲·嘧菌酯	AS	500~600 g/hm²	河北中保绿农作物科技有限公司
32.7%苯甲·嘧菌酯	AS	500~601 g/hm²	河北中保绿农作物科技有限公司

杀菌剂	剂型	推荐剂量	生产厂家
40%异菌脲	WP	1125~1500 g/hm²	富美实（中国)投资有限公司
80%代森锰锌	WP	3000~3750 g/hm²	青岛东生药业有限公司
250 g/L嘧菌酯	SC	720~900 mg/hm²	先正达（南通）公司
38%唑醚·啶酰菌（蓝楷）	SC	450~600 mg/hm²	河北中保绿农作物科技有限公司
32.5%苯甲·嘧菌酯（京彩）	SC	450~750 g/hm²	河北中保绿农作物科技有限公司
32.6%苯甲·嘧菌酯	AS	500~600 g/hm²	河北中保绿农作物科技有限公司
32.7%苯甲·嘧菌酯	AS	500~601 g/hm²	河北中保绿农作物科技有限公司

基因	引物名称	序列5’-3’
rDNA-ITS	ITS1	TCCGTAGGTGAACCTGCGG
rDNA-ITS	ITS4	TCCTCCGCT TATTGATATGC
EF-1α	EF1	ATGGGTAAGGA(A/G) GACAAGAC
EF-1α	EF2	GGA(G/A) GTACCAGT(G/C) ATCATGTT
ACT	512F	ATGTGCAAGGCCGGTTTCGC
ACT	738R	TACGAGTCCTTCTGGCCCAT
GAPDH	GDF	GCCGTCAACGACCCCTTCATTGA
GAPDH	GDR	GGGTGGAGTCGTACTTGAGCATGT

基因	引物名称	序列5’-3’
rDNA-ITS	ITS1	TCCGTAGGTGAACCTGCGG
rDNA-ITS	ITS4	TCCTCCGCT TATTGATATGC
EF-1α	EF1	ATGGGTAAGGA(A/G) GACAAGAC
EF-1α	EF2	GGA(G/A) GTACCAGT(G/C) ATCATGTT
ACT	512F	ATGTGCAAGGCCGGTTTCGC
ACT	738R	TACGAGTCCTTCTGGCCCAT
GAPDH	GDF	GCCGTCAACGACCCCTTCATTGA
GAPDH	GDR	GGGTGGAGTCGTACTTGAGCATGT

杀菌剂	有效浓度/(g/L)
杀菌剂	1	2	3
40%异菌脲	1	0.5	0.5
80%代森锰锌	0.625	0.9	1.8
250g/L嘧菌酯	0.025	0.1	0.4
38%唑醚·啶酰菌（蓝楷	0.025	0.1	0.4
32.5%苯甲·嘧菌酯（京彩）	0.025	0.1	0.4
3%甲霜·噁霉灵（秀苗）	0.025	0.1	0.4
40%嘧霉胺（施灰乐）	0.025	0.1	0.4