Abstract:

Panax notoginseng (Burk.) F.H. Chen is a perennial herbaceous medicinal plant of ginseng in Acanthopanax family. The study aims to isolate endophytic fungus strains which can produce the same or similar biological active substances as the host plant from the medicinal plant. P. notoginseng was used as material. The endophytic fungi strains of P. notoginseng was isolated and purified, and then the endophytic fungi strains were identified based on the traditional morphological characteristics and ITS sequence. A total of 24 strains of endophytic fungi were isolated from cultivated P. notoginseng, and they were identified as 12 genera (species). The types and numbers of endophytic fungi in different organs and tissues of cultivated P. notoginseng were quite different, the roots and leaves had the most, and the stems had less. Among them, 11 species and 6 genera were isolated from leaves, 1 species and 1 genus from stems, 12 species and 5 genera from roots. The statistical results showed that Penicillium commune and Colletotrichum gloeosporioides were the dominant species isolated from leaves, accounting for 27.28% of the isolates from leaves; the dominant strains isolated from the fibrous roots were Chaetomidium arxii, accounting for 72.73% of the isolated root strains. The cultivated P. notoginseng endophytic fungi have rich biodiversity, thus the experimental results could provide a material basis and experimental parameters for further exploring the relationship between cultivated P. notoginseng endophytic fungi and physiological active substances.

Key words: Panax notoginseng (Burk.) F.H. Chen, endophytic fungi, isolation and identification, ITS, medicinal plant