Abstract:

In order to isolate and identify pathogenic fungi of root rot disease in main ginseng producing areas of Liaoning Province, and provide theoretical support for subsequent ginseng disease resistance breeding and green control of root rot disease, by using tissue isolation technique and malt-agar method, the pathogens of ginseng root rot were isolated and purified and the pathogenicity was tested with 2-5 years old infected plants from serious disease areas of Xinbin. Combined with morphological observation, rDNA-ITS and EF-1α sequence analysis, morphological and molecular biological identification of the strains were carried out. Finally, 2 pathogenic fungi were isolated, with different colony morphology, named strain JS64-1 and strain JS64-3, which were identified as Fusarium solani and Fusarium oxysporum, respectively. The malt-agar method, which was used to determine the pathogenicity of ginseng root rot in this article, was convenient and time-saving, and the results were accurate and reliable. The study could lay a foundation for research on root diseases of other plants.

Key words: ginseng root rot, EF-1α, Fusarium solani, Fusarium oxysporum, malt-agar method, pathogenicity test