Biocontrol Fungus Coniothyrium minitans: Effects on Microbial Community Structure in Oilseed Rape Rhizosphere Soil

doi:10.11924/j.issn.1000-6850.casb2022-0381

Abstract

Abstract:

The aims are to determine the effect of Coniothyrium minitans on soil microbial community structure, and to provide a theoretical basis for the application of this biocontrol fungus in production. The occurrence of Sclerotinia stem rot was investigated by spraying C. minitans as treatment group (CM) and spraying clean water as control group (CK), and the changes of soil microbial community were analyzed by high-throughput sequencing technology. The results showed that the incidence rate and disease index of Sclerotinia stem rot in control group were up to 74.64% and 46.13, respectively. While, the incidence rate and disease index were reduced to 23.33% and 9.37, respectively, in treatment group, and the control effect was 79.70%. In addition, the soil microbial community structure was changed and the abundance and diversity of soil fungi were significantly increased after spraying C. minitans. At the phylum level, the relative abundance of Proteobacteria, Gemmatimonadetes, Ascomycota, Mortierellomycota and Basidiomycota were increased, but the relative abundance of Actinobacteria was decreased after spraying with C. minitans. At the genus level, the abundance of Sphingomonas, Lysobacter, Dokdonella, Trichocladium, Fusarium, Aspergillus and Plectosphaerella were increased, while, the relative abundance of unidentified_Chitinophagaceae, Stenotrophomonas, Geobacter, Thielavia and Cutaneotrichosporon were decreased after spraying with C. minitans. The application of C. minitans could effectively control Sclerotinia stem rot, increase the abundance and diversity of soil fungi, and improve soil microbial community structure. The results of this study can provide a basis for the large-scale promotion of C. minitans in the field.

Key words: Coniothyrium minitans, Sclerotinia sclerotiorum, Sclerotinia stem rot, Brassica napus, biological control fungi, microbial community

CLC Number:

S432.1

YANG Xiaoxiang, HUANG Xiaoqin, ZHANG Lei, ZHANG Zhongmei, XIAN Yunxi, ZHOU Xiquan, LIU Yong. Biocontrol Fungus Coniothyrium minitans: Effects on Microbial Community Structure in Oilseed Rape Rhizosphere Soil[J]. Chinese Agricultural Science Bulletin, 2022, 38(32): 92-98.

Figures/Tables 7

References 29

[1]	鲜赟曦, 张蕾, 杨潇湘, 等. 四川盆地栽培油菜品种菌核病抗性监测[J]. 中国农学通报, 2021, 37(6):117-122.
[2]	YANG X X, ZHANG L, XIANG Y J, et al. Comparative transcriptome analysis of Sclerotinia sclerotiorum revealed its response mechanisms to the biological control agent, Bacillus amyloliquefaciens[J]. Scientific reports, 2021, 11:5071. doi: 10.1038/s41598-021-84382-8 URL
[3]	李晓辉, 卢叶青, 吴明德, 等. 核盘菌菌核围微生物群落分析及其对盾壳霉重寄生的影响[J]. 植物病理学报, 2021, 51(2):258-267.
[4]	PARTRIDGE D E, SUTTON T B, JORDAN D L, et al. Management of Sclerotinia blight of peanut with the biological control agent Coniothyrium minitans[J]. Plant disease, 2006, 90:957-963. doi: 10.1094/PD-90-0957 URL
[5]	SMITH S N, PRINCE M, WHIPPS J M. Characterization of Sclerotinia and mycoparasite Coniothyrium minitans interaction by microscale co-culture[J]. Letters in applied microbiology, 2008, 47:128-133. doi: 10.1111/j.1472-765X.2008.02392.x URL
[6]	TOMPREFA N, MCQUILKEN M P, HILL R A, et al. Antimicrobial activity of Coniothyrium minitans and its macrolide antibiotic macrosphelide A[J]. Journal of applied microbiology, 2009, 106:2048-2056. doi: 10.1111/j.1365-2672.2009.04174.x URL
[7]	刘雪娇, 姚艳辉, 李红亚, 等. 贝莱斯芽胞杆菌3A3-15菌株对盆栽大豆土壤细菌群落结构的影响[J]. 农业生物技术学报, 2020, 33(1):77-86.
[8]	HUANG Z, LIU B, YIN Y, et al. Impact of biocontrol microbes on soil microbial diversity in ginger (Zingiber officinale Roscoe)[J]. Pest management science, 2021, 77(12):5537-5546. doi: 10.1002/ps.6595 URL
[9]	WANG X H, JI C, SONG X, et al. Biocontrol of two bacterial inoculant strains and their effects on the rhizosphere microbial community of field-grown wheat[J]. Biomed research international, 2021:8835275.
[10]	祝静, 于存. 长枝木霉菌肥对玉米生长、土壤肥力和根际微生物的影响[J]. 生物技术通报, 2022, 38(5):129-140.
[11]	伍文宪, 黄小琴, 张蕾, 等. 十字花科作物根肿病对根际土壤微生物群落的影响[J]. 生态学报, 2020, 40(5):1532-1541.
[12]	CAPORASO J G, LAUBER C L, WALTERS W A, et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample[J]. Proceedings of the national academy of sciences of the United States of America, 2011, 108(sup1):4516-4522.
[13]	ZINGER L, SHAHNAVAZ B, BAPTIST F, et al. Microbial diversity in alpine tundra soils correlates with snow cover dynamics[J]. The ISME journal, 2009, 3(7):850-859. doi: 10.1038/ismej.2009.20 URL
[14]	JIAO S, LIU Z S, LIN Y B, et al. Bacterial communities in oil contaminated soils: Biogeography and co-occurrence patterns[J]. Soil biology and biochemistry, 2016, 98:64-73. doi: 10.1016/j.soilbio.2016.04.005 URL
[15]	QIN J J, LI Y R, CAI Z M, et al. A metagenome-wide association study of gut microbiota in type 2 diabetes[J]. Nature, 2012, 490(7418):55-60. doi: 10.1038/nature11450 URL
[16]	MARTIN M. Cutadapt removes adapter sequences from high-throughput sequencing reads[J]. Embnet Journal, 2011, 17(1):10-12.
[17]	ASNICAR F, WEINGART G, TICKLE T L, et al. Compact graphical representation of phylogenetic data and metadata with GraPhlAn[J]. PeerJ, 2015, 3:e1029. doi: 10.7717/peerj.1029 URL
[18]	吕宁, 石磊, 刘海燕, 等. 生物药剂滴施对棉花黄萎病及根际土壤微生物数量和多样性的影响[J]. 应用生态学报, 2019, 30(2):602-614.
[19]	李叶青, 景张牧, 江皓, 等. 微生物组学及其在厌氧消化中的研究进展[J]. 生物技术通报, 2021, 37(1):90-101. doi: 10.13560/j.cnki.biotech.bull.1985.2020-1217
[20]	PANG G, CAI F, LI R X, et al. Trichoderma-enriched organic fertilizer can mitigate microbiome degeneration of monocropped soil to maintain better plant growth[J]. Plant and soil, 2017, 416:181-192. doi: 10.1007/s11104-017-3178-0 URL
[21]	DAVID C W, SUSAN D S, DAVID B R. The genus Sphingomonas: physiology and ecology[J]. Current opinion in biotechnology, 1996, 7:301-306. doi: 10.1016/S0958-1669(96)80034-6 URL
[22]	胡杰, 何晓红, 李大平, 等. 鞘氨醇单胞菌研究进展[J]. 应用与环境生物学报, 2007(3):431-437.
[23]	台喜生, 冯佳丽, 李梅, 等. 鞘氨醇单胞菌在生物降解方面的研究进展[J]. 湖南农业科学, 2011(4):21-25.
[24]	ADHIKARI T B, JOSEPH C M, YANG G, et al. Evaluation of bacteria isolated from rice for plant growth promotion and biological control of seedling disease of rice[J]. Canadian journal of microbiology, 2001, 47:916-924. pmid: 11718545
[25]	姬广海. 溶杆菌属及其在植物病害防治中的研究进展[J]. 云南农业大学学报, 2011, 26(1):124-130.
[26]	江北, 吕梦霞, 蒋冬花. 曲霉属真菌活性代谢产物及在农业生产中的应用研究进展[J]. 微生物学杂志, 2019, 39(2):103-110.
[27]	SHEMSHURA O N, BEKMAKHANOVA N E, MAZUNINA M N, et al. Isolation and identification of nematode-antagonistic compounds from the fungus Aspergillus candidus[J]. Fems microbiology letters, 2016, 363:1-9.
[28]	罗寒, 李晓栋, 李晓明, 等. 红树林来源内生真菌杂色曲霉Aspergillus versicolor MA-229次级代谢产物研究[J]. 中国抗生素杂志, 2017, 42(4):334-340.
[29]	赵莹. 根肿菌、寄主及根部内生微生物组交互作用研究[D]. 武汉: 华中农业大学, 2017.

处理	病株率/%	病情指数	病指防效/%
对照(CK)	74.67±2.49	46.13±0.70	—
盾壳霉(CM)	23.33±1.25	9.37±0.14	79.70

处理	病株率/%	病情指数	病指防效/%
对照(CK)	74.67±2.49	46.13±0.70	—
盾壳霉(CM)	23.33±1.25	9.37±0.14	79.70

项目	细菌		真菌
项目	盾壳霉(CM)	对照(CK)	盾壳霉(CM)	对照(CK)
OTU数	2176	2194	653	544
ACE指数	2553.757	2552.920	707.428	638.386
Chao1指数	2852.682	2851.983	693.355	625.493
香农指数	8.072	8.071	5.679	4.867
辛普森多样性指数	0.984	0.984	0.908	0.823
覆盖度	0.988	0.987	0.997	0.997

项目	细菌		真菌
项目	盾壳霉(CM)	对照(CK)	盾壳霉(CM)	对照(CK)
OTU数	2176	2194	653	544
ACE指数	2553.757	2552.920	707.428	638.386
Chao1指数	2852.682	2851.983	693.355	625.493
香农指数	8.072	8.071	5.679	4.867
辛普森多样性指数	0.984	0.984	0.908	0.823
覆盖度	0.988	0.987	0.997	0.997