Colonization of GFP-labelled Bacillus amyloliquefaciens Bam22 in Brassica napus

doi:10.11924/j.issn.1000-6850.casb2021-0830

Abstract

Abstract:

The paper aims to investigate the colonization of GFP-labelled Bacillus amyloliquefaciens Bam22 in Brassica napus and lay a solid foundation for the application of this biocontrol agent. In this study, plasmid pGFP4412 containing green fluorescent protein gene was introduced into Bam22 cells by natural transformation method to construct GFP fluorescent labelled strain. The growth curve and inhibition activity to pathogenic microorganism of wild type strain Bam22 and Bam22-GFP were compared. The number of colonized strains in different tissue parts of B. napus was observed, and the colonization rule and ability were analyzed. The results showed that the labelled strain Bam22-GFP emitted strong green fluorescence under the excitation light wavelength of 488 nm, and the GFP fluorescent protein labelling and its gene expression did not affect the growth curve and inhibition activity to pathogenic microorganism of Bam22. The labelled strain could colonize in the B. napus plant through the root irrigation, and was transmitted from the root to the stem and leaf of B. napus. The colonization rules of Bam22-GFP in the root, stem and leaf all showed a trend of first increase and then decrease. On the 45 ^th day after inoculation, the labelled strains could still be detected in the roots, stems and leaves of B. napus. The results show that Bam22 could colonize and transfer in B. napus by root irrigation and display good colonization ability, and it has a good application prospect in agricultural production.

Key words: Brassica napus, Bacillus amyloliquefaciens, green fluorescence protein labelling, colonization, Plasmodiophora brassicae

CLC Number:

S432.1

YANG Xiaoxiang, HUANG Xiaoqin, ZHANG Lei, ZHANG Zhongmei, XIAN Yunxi, ZHOU Xiquan, LIU Yong. Colonization of GFP-labelled Bacillus amyloliquefaciens Bam22 in Brassica napus[J]. Chinese Agricultural Science Bulletin, 2022, 38(1): 125-130.

Figures/Tables 6

References 25

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